H-Gly-Gly-Gly-OH是由三个甘氨酸残基通过肽键连接而成的三肽。
Cas No.:556-33-2
Sample solution is provided at 25 µL, 10mM.
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H-Gly-Gly-Gly-OH is a tripeptide composed of three glycine residues linked by peptide bonds. H-Gly-Gly-Gly-OH is widely used as a minimal model peptide to study backbone conformations and fundamental physicochemical properties due to no sidechain interference[1][2]. In CHO-PEPT1 cells, H-Gly-Gly-Gly-OH competitively inhibited the uptake of the peptide [³H]Gly-Sar, with an IC₅₀ value of 1.4±0.7mM[3]. The half-life of H-Gly-Gly-Gly-OH in rat plasma is 5min, and it is mainly absorbed by kidney tissue[4].
References:
[1] Guo M, et al. Triglycine (GGG) adopts a polyproline II (pPII) conformation in its hydrated crystal form: revealing the role of water in peptide crystallization. J Phys Chem Lett. 2021;12(23):5617-5622.
[2] Zhang Y, et al. The effect of chain length and side chains on the solubility of peptides in water from 278.15 K to 313.15 K: a case study in glycine homopeptides and dipeptides. Fluid Phase Equilib. 2022;556:113375.
[3] Covitz KM, Amidon GL, Sadée W. Human dipeptide transporter, hPEPT1, stably transfected into Chinese hamster ovary cells. Pharm Res. 1996;13(11):1631-1634.
[4] Adibi SA, Morse EL. Enrichment of glycine pool in plasma and tissues by glycine, di-, tri-, and tetraglycine. Am J Physiol. 1982;243(5):E413-E417.
H-Gly-Gly-Gly-OH是由三个甘氨酸残基通过肽键连接而成的三肽。由于无侧链干扰，H-Gly-Gly-Gly-OH被广泛用作研究主链构象和基本理化性质的最小模型肽。在CHO-PEPT1细胞中，H-Gly-Gly-Gly-OH竞争性地抑制了肽链[³H]Gly-Sar的摄取，IC50值为1.4±0.7mM[3]。H-Gly-Gly-Gly-OH在大鼠血浆中的半衰期为5min，并主要被肾脏组织吸收[4]。
| Animal experiment [1]: | |
Animal models | Male Sprague-Dawley rats; 270-300g |
Preparation Method | The right jugular vein was injected with one of the following test solutions: 150mM NaCl, 300mM glycine, 150mM diglycine, 100mM H-Gly-Gly-Gly-OH (triglycine), or 75mM tetraglycine. Plasma glutathione concentrations were measured at 2, 5, 10, 15, 20, and 30 minutes after injection. The concentrations of glutathione in the liver, muscle, and kidney were measured at 5 and 30 minutes after injection. |
Dosage form | 1μmol/g; i.v. |
Applications | The changes in plasma glycine concentrations after H-Gly-Gly-Gly-OH injection was characterized by a sharp in-crease (over tenfold) at 2min followed by a sharp de-crease at 5min, at 30min the concentration was still considerably greater than thebasal level (over a twofold increase). Among the glycine peptides, each increase in the number of glycine residues resulted in significantly lower initial glycine concentration. The concentration of glycine in the muscle was not significantly affected by injections. The increase in glycine concentration 5min after the injections was always considerably greater in the kidney than in the liver. |
References: | |
| Cas No. | 556-33-2 | SDF | |
| 别名 | 甘氨酰-甘氨酰-甘氨酸 | ||
| 分子式 | C6H11N3O4 | 分子量 | 189.17 |
| 溶解度 | H2O : 50 mg/mL (264.31 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
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| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.2863 mL | 26.4313 mL | 52.8625 mL |
| 5 mM | 1.0573 mL | 5.2863 mL | 10.5725 mL |
| 10 mM | 528.6 μL | 2.6431 mL | 5.2863 mL |
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动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
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| % DMSO % % Tween 80 % saline | ||||||||||
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计算结果:
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2.
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