H-Gly-Gly-Gly-OH is a tripeptide composed of three glycine residues linked by peptide bonds. H-Gly-Gly-Gly-OH is widely used as a minimal model peptide to study backbone conformations and fundamental physicochemical properties due to no sidechain interference[1][2]. In CHO-PEPT1 cells, H-Gly-Gly-Gly-OH competitively inhibited the uptake of the peptide [³H]Gly-Sar, with an IC₅₀ value of 1.4±0.7mM[3]. The half-life of H-Gly-Gly-Gly-OH in rat plasma is 5min, and it is mainly absorbed by kidney tissue[4].
References:
[1] Guo M, et al. Triglycine (GGG) adopts a polyproline II (pPII) conformation in its hydrated crystal form: revealing the role of water in peptide crystallization. J Phys Chem Lett. 2021;12(23):5617-5622.
[2] Zhang Y, et al. The effect of chain length and side chains on the solubility of peptides in water from 278.15 K to 313.15 K: a case study in glycine homopeptides and dipeptides. Fluid Phase Equilib. 2022;556:113375.
[3] Covitz KM, Amidon GL, Sadée W. Human dipeptide transporter, hPEPT1, stably transfected into Chinese hamster ovary cells. Pharm Res. 1996;13(11):1631-1634.
[4] Adibi SA, Morse EL. Enrichment of glycine pool in plasma and tissues by glycine, di-, tri-, and tetraglycine. Am J Physiol. 1982;243(5):E413-E417.
H-Gly-Gly-Gly-OH是由三个甘氨酸残基通过肽键连接而成的三肽。由于无侧链干扰，H-Gly-Gly-Gly-OH被广泛用作研究主链构象和基本理化性质的最小模型肽。在CHO-PEPT1细胞中，H-Gly-Gly-Gly-OH竞争性地抑制了肽链[³H]Gly-Sar的摄取，IC50值为1.4±0.7mM[3]。H-Gly-Gly-Gly-OH在大鼠血浆中的半衰期为5min，并主要被肾脏组织吸收[4]。