H-D-Pro-Phe-Arg-pNA . 2 HCl is a chromogenic peptide substrate that has been used for the determination of kallikreins [1]. H-D-Pro-Phe-Arg-pNA . 2 HCl can be cleaved by Kallikreins, releasing p-nitroaniline (pNA), and the release rate is determined by spectrophotometry at 405nm[2]. H-D-Pro-Phe-Arg-pNA . 2 HCl can serve as a substrate for bovine trypsin and be used for screening irreversible serine protease inhibitors[3].
References:
[1] Shibuya Y, Yamamoto T, Tanaka H, et al. Mechanized assay of plasma prekallikrein by activation with Pseudomonas aeruginosa elastase and amidolysis of chromogenic substrate[J]. Clinica chimica acta, 1991, 200(2-3): 119-127.
[2] Shin I S, Shim Y B, Hong C M, et al. An improved, reliable and practical kinetic assay for the detection of prekallikrein activator in blood products[J]. Archives of pharmacal research, 2002, 25(4): 505-510.
[3] Zhao Y, Jin Y, Wei S S, et al. Purification and characterization of an irreversible serine protease inhibitor from skin secretions of Bufo andrewsi[J]. Toxicon, 2005, 46(6): 635-640.
H-D-Pro-Phe-Arg-pNA . 2 HCl是一种显色肽底物，已用于Kallikrein活性的测定[1]。H-D-Pro-Phe-Arg-pNA . 2 HCl可被Kallikrein裂解，释放出对硝基苯胺(pNA)，释放速率可通过分光光度法在405nm波长处进行测定[2]。H-D-Pro-Phe-Arg-pNA . 2 HCl可作为牛胰蛋白酶的底物，并用于筛选不可逆的丝氨酸蛋白酶抑制剂[3]。