GlpBio

Bz-Nle-Lys-Arg-Arg-AMC

目录号: GA21221纯度: >98.00%同义词: Benzoyl-Nle-Lys-Arg-Arg-AMC, Benzoyl-Nle-Lys-Arg-Arg-7-amino-4-methylcoumarin, Bz-Nle-Lys-Arg-Arg-AMC
COASDSData SheetHPLC

Bz-Nle-Lys-Arg-Arg-AMC 是黄热病病毒 (YFV) 非结构 3 (NS3)、登革热病毒 (DV) NS2B/3 丝氨酸蛋白酶和寨卡病毒的荧光四肽底物(ZIKV) NS2B/NS3 丝氨酸蛋白酶。

Bz-Nle-Lys-Arg-Arg-AMC
Cas No.: 863975-32-0
规格价格库存数量操作
1mg¥392.00现货
1
5mg¥1,714.00现货
1
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产品描述 Description

Bz-Nle-Lys-Arg-Arg-AMC is a fluorogenic tetra-peptide substrate for yellow fever virus (YFV) non-structural 3 (NS3), dengue virus (DV) NS2B/3 serine proteases, and Zika virus (ZIKV) NS2B/NS3 serine proteases [1,2,3].

After enzymatic hydrolysis by YNS2B/NS3 serine proteases, Bz-Nle-Lys-Arg-Arg-AMC releases 7-amino-4-methylcoumarin (AMC), whose fluorescence can be used to determine the activity of YNS2B/NS3 serine proteases. AMC exhibits excitation/emission maxima at 340-360/440-460 nm, respectively.

References:
[1]. Ulanday GE, Okamoto K, Morita K. Development and utility of an in vitro, fluorescence-based assay for the discovery of novel compounds against dengue 2 viral protease. Tropical Medicine and Health. 2016 Dec;44:1-0.
[2]. Loehr K, Knox JE, Phong WY, Ma NL, Yin Z, Sampath A, Patel SJ, Wang WL, Chan WL, Rao KR, Wang G. Yellow fever virus NS3 protease: peptide-inhibition studies. Journal of general virology. 2007 Aug;88(8):2223-7.
[3]. Lee H, Ren J, Nocadello S, Rice AJ, Ojeda I, Light S, Minasov G, Vargas J, Nagarathnam D, Anderson WF, Johnson ME. Identification of novel small molecule inhibitors against NS2B/NS3 serine protease from Zika virus. Antiviral research. 2017 Mar 1;139:49-58.

Bz-Nle-Lys-Arg-Arg-AMC 是黄热病病毒 (YFV) 非结构 3 (NS3)、登革热病毒 (DV) NS2B/3 丝氨酸蛋白酶和寨卡病毒的荧光四肽底物(ZIKV) NS2B/NS3 丝氨酸蛋白酶 [1,2,3]。

在 YNS2B/NS3 丝氨酸蛋白酶酶促水解后，Bz-Nle-Lys-Arg-Arg-AMC 释放 7-amino-4 -甲基香豆素 (AMC)，其荧光可用于确定 YNS2B/NS3 丝氨酸蛋白酶的活性。 AMC 的激发/发射最大值分别位于 340-360/440-460 nm。

实验参考方法 Experimental Reference Method

Cell experiment [1]:

Preparation Method

FRET assay was performed to evaluate NS2B-NS3 protease activity and its inhibition by flavonoids. NS2B-NS3 protease in buffer solution (20 mM HEPES, 20 % glycerol and 1 mM CHAPS, pH 9.0) was pre-incubated at 37 °C for 30 min, and the reaction was initiated by adding 100 μM of the substrate Bz-Nle-Lys-Arg-Arg-AMC. The fluorescence was monitored per 15 s in 10 min with a microplate spectrophotometer at 360 nm and 450 nm for excitation and emission, respectively.

The inhibition assay was performed in 100 μL of buffer containing 20 mM HEPES (pH 9.0), 20 % glycerol, 1 mM CHAPS, 0.1 μM NS2B-NS3 protease and 100 μM substrate with varying concentrations of inhibitors. NS2B-NS3 protease was pre-incubated with inhibitors at 37°C for 30 min, and the reaction was initiated by adding 100 μM of the substrate Bz-Nle-Lys-ArgArg-AMC. After 10 min, the fluorescence was monitored with a microplate spectrophotometer at 360 nm and 450 nm for excitation and emission, respectively. Aprotinin (1 μM) was used as a positive control.

Reaction Conditions

100 µM for 10 min

References:

[1]: Zou M, Liu H, Li J, Yao X, Chen Y, Ke C, Liu S. Structure-activity relationship of flavonoid bifunctional inhibitors against Zika virus infection. Biochemical pharmacology. 2020 Jul 1;177:113962.

产品文档 Product Documents

Purity:>98.00%
COASDSData SheetHPLC

化学性质Chemical Properties

CAS 号
863975-32-0
同义词
Benzoyl-Nle-Lys-Arg-Arg-AMC, Benzoyl-Nle-Lys-Arg-Arg-7-amino-4-methylcoumarin, Bz-Nle-Lys-Arg-Arg-AMC
分子式
C41H60N12O7
分子量
833 g/mol
溶解性
DMF: 30mg/mL,DMSO: 30mg/mL,DMSO:PBS (pH 7.2) (1:1): 0.5mg/mL,Ethanol: 20mg/mL
保存条件
Store at -20°C
General tips
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
为了提高溶解度，请将管子加热至 37°C，然后在超声波浴中震荡一段时间。
Shipping Condition
评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备 RT，或根据请求配备蓝冰。

计算工具摩尔浓度 / 稀释 / 分子量 / 单位换算 / 体内配方 / 溶解度

g/mol