Fluorescein Tyramide is a green fluorescent labeling reagent (λabs: 494nm; λem: 517nm). Under the catalysis of horseradish peroxidase (HRP), Fluorescein Tyramide enables in situ signal deposition via tyramide signal amplification (TSA) technology, enhancing detection sensitivity by 10–100 times, making Fluorescein Tyramide suitable for low-abundance target detection. Fluorescein Tyramide can be used in related research such as immunohistochemistry (IHC), fluorescence in situ hybridization (FISH), and flow cytometry (FCM)[1-2].
References:
[1] Hopman AH, Ramaekers FC, Speel EJ. Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for In situ hybridization using CARD amplification. J Histochem Cytochem. 1998 Jun;46(6):771-7.
[2] van Gijlswijk RP, Zijlmans HJ, Wiegant J, et al. Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization. J Histochem Cytochem. 1997 Mar;45(3):375-82.
Fluorescein Tyramide是一种绿色荧光标记试剂（λabs: 494nm; λem: 517nm）。Fluorescein Tyramide可在辣根过氧化物酶（HRP）催化下通过酪胺信号放大（TSA）技术实现信号原位沉积，使检测灵敏度提升10-100倍，适用于低丰度靶标检测。Fluorescein Tyramide可用于免疫组化（IHC）、原位杂交（FISH）和流式细胞术（FCM）等相关研究[1-2]。