Dox-btn2 is a biotinylated derivative of Doxorubicin , with a biotin label at the point of conjugation to doxorubicin at 3'-NH2. Dox-btn2 can be used for cell imaging. While Doxorubicin is mainly accumulated in the nucleus, while Dox-btn2 is mainly located in the cytoplasm.
Dox-btn2 (1 μM; 6 h; Exc=531/40 nm and Emi=593/40 nm) exhibits visualization in U2OS cells, helps in situ mapping with Chem-map of small-molecule interactions with DNA and chromatin proteins[1].
References:
[1]. Yu Z, et al. Chem-map profiles drug binding to chromatin in cells. Nat Biotechnol. 2023 Jan 23.