DiIC1(5) is a signal-off fluorescent probe for the detection of mitochondrial membrane potential disruption, with excitation/emission of 638/675nm, respectively [1]. DiIC1(5) penetrates the plasma membrane of eukaryotic cells and accumulates primarily in mitochondria with active membrane potentials, and a breakdown of the mitochondrial membrane potential reflects cell death and is detected by a drop in the DiIC1(5) signal [2]. DiIC1(5) has been widely used in multichannel flow cytometry to study the activation of apoptosis in sperm[3]. DiIC1(5) can be used to develop the novel flow cytometry assay to measure mitochondrial reactive oxygen intermediates (ROI) production and to study the functional integrity of respiratory chain complexes at the single-cell level[4].
References:
[1] Munoz L E, Maueröder C, Chaurio R, et al. Colourful death: Six-parameter classification of cell death by flow cytometry—Dead cells tell tales[J]. Autoimmunity, 2013, 46(5): 336-341.
[2] Janko C, Munoz L, Chaurio R, et al. Navigation to the graveyard-induction of various pathways of necrosis and their classification by flow cytometry[M]//Necrosis: Methods and Protocols. Totowa, NJ: Humana Press, 2013: 3-15.
[3] Kim H H, Funaro M, Mazel S, et al. Flow cytometric characterization of apoptosis and chromatin damage in spermatozoa[J]. Reproductive BioMedicine Online, 2013, 26(4): 393-395.
[4] Pham N A, Robinson B H, Hedley D W. Simultaneous detection of mitochondrial respiratory chain activity and reactive oxygen in digitonin‐permeabilized cells using flow cytometry[J]. Cytometry: The Journal of the International Society for Analytical Cytology, 2000, 41(4): 245-251.
DiIC1(5)是一种用于检测线粒体膜电位破坏的信号关闭型荧光探针，激发/发射波长分别为638nm和 675nm[1]。DiIC1(5)能够穿透真核细胞的质膜，并主要积聚在具有活跃膜电位的线粒体中；线粒体膜电位的崩溃反映了细胞死亡，可通过DiIC1(5)信号的下降来检测[2]。DiIC1(5)已被广泛用于多通道流式细胞术中，以研究精子凋亡的激活过程[3]。DiIC1(5)可用于开发新型流式细胞检测方法，以测量线粒体活性氧中间体的产生，并在单细胞水平上研究呼吸链复合物的功能完整性[4]。