Dihydrofolic acid is an active metabolite of folic acid and an intermediate in the biosynthesis of both purines and pyrimidines.1,2 It is formed via the NADH-dependent reduction of folic acid by dihydrofolate reductase (DHFR).2 Dihydrofolic acid (10, 50, and 100 ?g/ml) reduces insulin-like growth factor 1 receptor (IGF-1R) promotor activity in a cell-based reporter assay.3
1.Futterman, S.Enzymatic reduction of folic acid and dihydrofolic acid to tetrahydrofolic acidJ. Biol. Chem.228(2)1031-1038(1957) 2.López, C.A., and Menendez, J.C.AntimetabolitesMedicinal Chemistry of Anticancer Drugs9-52(2008) 3.Attias, Z., Werner, H., and Vaisman, N.Folic acid and its metabolites modulate IGF-I receptor gene expression in colon cancer cells in a p53-dependent mannerEndocr. Relat. Cancer13(2)571-581(2006)