Dexamethasone (DHAP)

Cell lines

Human osteosarcoma cell lineMG-63

Preparation method

The solubility of this compound in DMSO is >19.6mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

100 nM; 0-36 h

Applications

In human osteoblast-like cell lineMG-63, Dexamethasone (Dex) exhibited dose-dependently upregulating RhoB protein expression. Dex treatment for above 2 days could significantly inhibit the growth of MG-63 cells in a dose dependent way.

Animal models

LPS-induced neuroinflammation mice

Dosage form

0.25 mg/kg; intravenous (IV) or intranasal (IN).

Application

In LPS-induced neuroinflammation mice, IN administration of DX significantly reduced the level of IL-6 in the brain extracts and reduced the percentage of GFAP+ brain cells one day after treatment. Compared with IV route, higher levels of DX are observed in the cerebrovasculature of mice after IN administration.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Diao F1, Chen K2,3, Wang Y1, et al. Involvement of small G protein RhoB in the regulation of proliferation, adhesion and migration by dexamethasone in osteoblastic cells. PLoS One. 2017 Mar 21;12(3):e0174273.

[2]. Meneses G1, Gevorkian G1, Florentino A1, et al. INTRANASAL DELIVERY OF DEXAMETHASONE EFFICIENTLY CONTROLS LPS-INDUCED MURINE NEUROINFLAMMATION. Clin Exp Immunol. 2017 Jul 28.