ddATP trisodium is a nucleotide analog used in molecular biology and biochemistry, primarily for DNA sequencing and polymerase chain reactions (PCR)[1]. ddATP trisodium is a modified nucleotide analog in which the 3'-hydroxyl group of the 2'-deoxyribose sugar is replaced by a hydrogen atom[2]. This modification prevents the formation of phosphodiester bonds during DNA synthesis, thereby effectively terminating the elongation of the DNA chain[3]. Some studies also suggest that ddATP trisodium can inhibit viral replication[4].
In vitro, treating oocytes with 100μM ddATP trisodium for 5 hours and inducing nuclear DNA double-strand breaks with 0.5μM bleomycin for 1 hour, ddATP trisodium can effectively inhibit mitochondrial DNA (mtDNA) replication in oocytes and reduce γH2AX foci in oocytes[5]. Additionally, studies have found that ddATP trisodium (at concentrations of 1, 2, 4, and 8μmol/mL) can synergistically inhibit the growth of Corynebacterium pseudotuberculosis with penicillin and decrease the minimum inhibitory concentration (MIC) of penicillin against Corynebacterium pseudotuberculosis[6].
References:
[1] Green MR, Sambrook J. Labeling the 3' Termini of Oligonucleotides Using Terminal Deoxynucleotidyl Transferase. Cold Spring Harb Protoc. 2021 Aug 2;2021(8).
[2] Perry CM, Noble S. Didanosine: an updated review of its use in HIV infection. Drugs. 1999 Dec;58(6):1099-135.
[3] Yousaf SI, Carroll AR, Clarke BE. A new and improved method for 3'-end labelling DNA using [alpha-32P]ddATP. Gene. 1984 Mar;27(3):309-13.
[4] Cronn RC, Remington KM, Preston BD, et al. Inhibition of reverse transcriptase from feline immunodeficiency virus by analogs of 2'-deoxyadenosine-5'-triphosphate. Biochem Pharmacol. 1992 Oct 6;44(7):1375-81.
[5] Ma JY, Feng X, Xie FY, et al. Double-strand breaks induce short-scale DNA replication and damage amplification in the fully grown mouse oocytes. Genetics. 2021 Jun 24;218(2):iyab054.
[6] Bernardino PN, de Paula CL, Pereira AFM, et al. Potential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosis. Braz J Microbiol. 2023 Mar;54(1):559-563.
ddATP trisodium 是一种用于分子生物学和生物化学的核苷酸类似物，主要用于 DNA 测序和聚合酶链反应（PCR）[1]。ddATP trisodium 是一种经过修饰的核苷酸类似物，其 2'-脱氧核糖的 3'-羟基被氢原子取代[2]。这种修饰使得 DNA 合成过程中无法形成磷酸二酯键，从而有效终止 DNA 链的延伸[3]。也有研究认为ddATP trisodium可抑制病毒的复制[4]。
在体外，用100μM ddATP trisodium处理卵母细胞5小时，用0.5μM博来霉素诱导核DNA双链断裂1小时，ddATP trisodium可有效抑制卵母细胞的线粒体DNA(mtDNA)复制，并且ddATP trisodium降低卵母细胞的γH2AX病灶[5]。也有研究发现ddATP trisodium（1、2、4、8μmol/mL）可协同青霉素抑制假结核棒状杆菌的生长能力，并降低假结核棒状杆菌对青霉素的最小抑菌浓度（MIC）[6]。