D-Fructose-6-phosphate (sodium salt) is an endogenous metabolite and an intermediate in glycolysis and the pentose phosphate pathway[1, 2]. D-Fructose-6-phosphate is produced from glucose-6-phosphate by the enzyme phosphoglucose isomerase and can be further converted to fructose-1,6-bisphosphate by fructose-1,6-bisphosphate kinase, thus driving glycolysis forward[3]. The key role of D-Fructose-6-phosphate lies in its function as a central hub in energy metabolism and biosynthetic pathways; it is commonly used in studies of cellular metabolism, enzyme kinetics, and in vitro reconstitution of metabolic pathways[4, 5].
References:
[1] Severin S E, Stepanova N G. Interrelationship between glycolysis and the anaerobic part of the pentose phosphate pathway of carbohydrate metabolism in the myocardium[J]. Advances in Enzyme Regulation, 1981, 19: 235-255.
[2] Cronín C N, Nolan D P, Voorheis H P. The enzymes of the classical pentose phosphate pathway display differential activities in procyclic and bloodstream forms of Trypanosoma brucei[J]. FEBS letters, 1989, 244(1): 26-30.
[3] Fan L, Chen J, Chen Y, et al. Recent Advances on Biological Production of a Functional Low-Calorie Sugar d-Tagatose[J]. Journal of Agricultural and Food Chemistry, 2025, 73(30): 18511-18524.
[4] Okar D A, Lange A J. Fructose‐2, 6‐bisphosphate and control of carbohydrate metabolism in eukaryotes[J]. Biofactors, 1999, 10(1): 1-14.
[5] Müller S, Zimmermann F K, Boles E. Mutant studies of phosphofructo-2-kinases do not reveal an essential role of fructose-2, 6-bisphosphate in the regulation of carbon fluxes in yeast cells[J]. Microbiology, 1997, 143(9): 3055-3061.
D-Fructose-6-phosphate (sodium salt)是一种内源性代谢物，是糖酵解和磷酸戊糖途径的中间产物[1, 2]。D-Fructose-6-phosphate由葡萄糖-6-磷酸通过磷酸葡萄糖异构酶催化转化而来，并可在磷酸果糖激酶-1的催化下进一步转化为果糖-1,6-二磷酸，从而推动糖酵解的进行[3]。D-Fructose-6-phosphate的核心作用在于作为能量代谢和生物合成代谢的枢纽，常用于细胞代谢研究、酶动力学分析以及体外重建代谢通路的实验体系中[4, 5]。