CycLuc1 is a synthetic, brain-permeable luciferase substrate, with maximum emission wavelength λmax=604nm[1]. CycLuc1 can be used for fluorescence imaging analysis in vivo and in vitro characterization studies of mutant luciferase[2].
CycLuc1 has been used to monitor intracranial glioblastoma (GBM) xenograft tumor growth in athymic nude mice at doses of 5-25mg/kg[3]. Intraperitoneal injection of CycLuc1 at 15mg/kg significantly enhanced in vivo bioluminescence imaging of gene expression in the sub-forniceal organ (SFO) or hypothalamic paraventricular nucleus (PVN) within male C57BL/6 mice (8-10 week of age) [4]. After intravenous (iv) injection of CycLuc1 (5mmol/L) in Pathogen-free BALB/c, FVB/N, C57BL/6 and luciferase-expressing transgenic mice (FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ), the CycLuc1 signal peaked 4-5 minutes and then produced a stable signal that lasted for more than 60 minutes[5].
References:
[1] Kuchimaru T, Iwano S, Kiyama M, et al. A luciferin analogue generating near-infrared bioluminescence achieves highly sensitive deep-tissue imaging[J]. Nature communications, 2016, 7(1): 11856.
[2] Harwood K R, Mofford D M, Reddy G R, et al. Identification of mutant firefly luciferases that efficiently utilize aminoluciferins[J]. Cell Chemical Biology, 2011, 18(12): 1649-1657.
[3] Gupta S K, Kim M, Marin B M, et al. Synthetic luciferin, CycLuc1, improves bioluminescence imaging for intracranial glioblastoma xenografts[J]. Cancer Research, 2018, 78(13_Supplement): 4112-4112.
[4] Simonyan H, Hurr C, Young C N. A synthetic luciferin improves in vivo bioluminescence imaging of gene expression in cardiovascular brain regions[J]. Physiological Genomics, 2016, 48(10): 762-770.
[5] Evans M S, Chaurette J P, Adams Jr S T, et al. A synthetic luciferin improves bioluminescence imaging in live mice[J]. Nature methods, 2014, 11(4): 393-395.
CycLuc1是一种合成的、具有血脑屏障通透性的荧光素酶底物，最大发射波长λmax=604nm[1]。CycLuc1可用于体内荧光成像分析以及突变荧光素酶的体外表征研究[2]。
CycLuc1以5-25mg/kg的剂量已被用于在无胸腺裸鼠中监测颅内胶质母细胞瘤（GBM）异种移植肿瘤的生长[3]。在8-10周龄的雄性C57BL/6小鼠中，腹腔注射15mg/kg CycLuc1显著增强了穹窿下器官（SFO）或下丘脑室旁核（PVN）基因表达的体内生物发光成像效果[4]。在无特定病原体的BALB/c、FVB/N、C57BL/6和表达荧光素酶的转基因小鼠（FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ）中静脉注射CycLuc1（5mmol/L）后，CycLuc1信号在4-5分钟达到峰值，随后产生持续超过60分钟的稳定信号[5]。