Recombinant Coronavirus 2019 3CL Protease having a Mw of approximately 33.8 kDa was purified from E. coli.The CoV-2 3CL contains 306 amino acids and purified by proprietary chromatographic technique.
CoV-2 3CL
目录号: GP26423纯度: >98%
Recombinant Coronavirus 2019 3CL Protease having a Mw of approximately 33
| 规格 | 价格 | 库存 | 数量 | 操作 |
|---|---|---|---|---|
| 100μg | ¥7,000.00 | 现货 | 1 | |
| 0.25mg | ¥12,600.00 | 现货 | 1 | |
| 1mg | ¥35,000.00 | 现货 | 1 |
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Cell187(9):2288-2304 (2024)
Cell183(7):1867-1883 (2020)
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Purity:>98%
Product Data
Purity
Protein is >98% pure as determined by SDS-PAGE and HPLC analyses.
Source
Escherichia Coli.
Physical Appearance
Filtered White lyophilized (freeze-dried) powder.
Shipping Condition
atRoomtemp.
Amino Acid Sequence
SGFRKMAFPS GKVEGCMVQV TCGTTTLNGL WLDDVVYCPR HVICTSEDML NPNYEDLLIR KSNHNFLVQA GNVQLRVIGH SMQNCVLKLK VDTANPKTPK YKFVRIQPGQ TFSVLACYNG SPSGVYQCAM RPNFTIKGSF LNGSCGSVGF NIDYDCVSFC YMHHMELPTG VHAGTDLEGN FYGPFVDRQT AQAAGTDTTI TVNVLAWLYA AVINGDRWFL NRFTTTLNDF NLVAMKYNYE PLTQDHVDIL GPLSAQTGIA VLDMCASLKE LLQNGMNGRT ILGSALLEDE FTPFDVVRQC SGVTFQ
Solubility
It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. CoV-2 3CL is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Stability
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Biological Activity
CoV-2 3CL is a cysteine protease that cleaves proteins with sequences including LQ(S/A/G). The cleavage takes c-terminal to the glutamine residue.
Formulation
CoV-2 3CL solution contains 5 % Trehalose, 0.02 % Tween-20 and PBS, pH 7.4.