cGAMP (Cyclic AMP-GMP)是一种环状二核苷酸，是哺乳动物细胞内的第二信使，是在细胞受到威胁时“即时”合成的。
Cas No.:849214-04-6
Sample solution is provided at 25 µL, 10mM.
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cGAMP (Cyclic AMP-GMP) is a cyclic dinucleotide that acts as a second messenger in mammalian cells and is synthesized "on demand" when cells are threatened[1, 2]. cGAMP activates the stimulator of interferon genes (STING), triggering a signaling cascade that leads to the production of type I interferons and other immune mediators[3]. Under cGAMP stimulation, cells show increased IFNB1 transcription, but no abnormal transcription of genes encoding interleukin-1 (IL1), interleukin-6 (IL6), or tumor necrosis factor (TNF)[4].
In vitro, treatment of mouse bone marrow-derived dendritic cells and human peripheral blood mononuclear cell-derived dendritic cells with cGAMP (5mg/mL, 60mg/mL) for 24h directly activated both mouse and human dendritic cells. The purity of CD11c+ cells in the tested cell populations was 95% for human peripheral blood mononuclear cell-derived dendritic cells and 82% for mouse bone marrow-derived dendritic cells[5].
In vivo, cGAMP (5μg) administered as a nasal mucosal adjuvant to immunized mice promoted antigen-specific proliferation of mouse splenocytes and enhanced antigen-specific humoral immune responses[5].
References:
[1] Liu Y, Fei Y, Wang X, et al. Biomaterial-enabled therapeutic modulation of cGAS-STING signaling for enhancing antitumor immunity[J]. Molecular Therapy, 2023, 31(7): 1938-1959.
[2] Su Y. Development of Riboswitch-based Sensors for High-throughput Enzyme Activity Screens[M]. University of California, Berkeley, 2018.
[3] Kaushal A. A central role of stimulator of interferon genes’ adaptor protein in defensive immune response[J]. Immunologic Research, 2025, 73(1): 39.
[4] Liu Y, Jesus A A, Marrero B, et al. Activated STING in a vascular and pulmonary syndrome[J]. New England Journal of Medicine, 2014, 371(6): 507-518.
[5] Škrnjug I, Guzmán C A, Ruecker C. Cyclic GMP-AMP displays mucosal adjuvant activity in mice[J]. PloS one, 2014, 9(10): e110150.
cGAMP (Cyclic AMP-GMP)是一种环状二核苷酸，是哺乳动物细胞内的第二信使，是在细胞受到威胁时“即时”合成的[1, 2]。cGAMP (Cyclic AMP-GMP)激活干扰素基因刺激因子（STING），激活导致产生I型干扰素和其他免疫介质的信号级联[3]。在cGAMP (Cyclic AMP-GMP)刺激下，细胞显示IFNB1转录增加，但编码白介素-1（IL1）、白介素-6（IL6）或肿瘤坏死因子（TNF）基因的转录无异常[4]。
在体外，cGAMP (Cyclic AMP-GMP)（5mg/mL, 60mg/mL）处理小鼠骨髓来源树突状细胞和人外周血单核细胞来源树突状细胞24h，直接激活了小鼠和人树突状细胞。测试细胞群体中CD11c+细胞的纯度，人外周血单核细胞来源的树突状细胞为95%，小鼠骨髓来源的树突状细胞为82%[5]。
在体内，cGAMP (Cyclic AMP-GMP)（5μg）通过鼻黏膜佐剂处理免疫小鼠，促进了小鼠脾细胞的抗原特异性增殖能力，促进了抗原特异性体液免疫反应[5]。
| Cell experiment [1]: | |
Cell lines | Murine bone marrow-derived dendritic cells, human PBMC-derived dendritic cells |
Preparation Method | Murine bone marrow-derived dendritic cells (DCs) and human PBMC-derived DCs were stimulated in vitro with c-di-AMP, cGAMP (both at 5mg/mL or 60mg/mL) or left untreated (mock) for 24h. The dendritic cells were decorated with fluorophore-conjugated antibodies against the DC activation markers CD40, CD54, CD80, CD83, CD86 or MHC class II (I-Ab) and analyzed by flow cytometry. |
Reaction Conditions | 5mg/mL, 60mg/mL; 24h |
Applications | cGAMP directly activates murine and human dendritic cells in vitro. The purity of the tested cell populations with respect to CD11c+ cells was 95% for the human PBMC derived DCs and 82% for the mouse bone marrow-derived DCs. |
| Animal experiment [1]: | |
Animal models | Female C57BL/6 (H-2b) mice |
Preparation Method | Mouse immunization experiments Five animals per group were immunized intra-nasal (i. n.) on days 0, 14 and 28. Animals were anesthetized with Isoflurane and treated 10mL per nostril with 15μg ovalbumin (OVA) alone or co-adminis tered with 5μg per dose of c-di-AMP, cGAMP or cholera toxin B subunit (CTB) in Ampuwa or with Ampuwa alone in the control group (mock immunization). On day 42 after immunization animals were sacrificed and samples were collected. |
Dosage form | 5μg; intra-nasal (i. n.) |
Applications | The cGAMP-dependent enhanced specific IgG and IgA titers in our mouse immunization experiments suggest that the use of cGAMP promotes the antigen-specific humoral immune response. Spleen cells from mice immunized with cGAMP-adjuvanted antigen showed a facilitated antigen-specific proliferation capacity. |
References: | |
| Cas No. | 849214-04-6 | SDF | |
| 别名 | Cyclic GMP-AMP; 3',3'-cGAMP | ||
| Canonical SMILES | O=P(O[C@H]1[C@@H](O)[C@H](N2C=NC3=C2N=CN=C3N)O[C@@H]1COP4(O)=O)(O)OC[C@@H]5[C@@H](O4)[C@@H](O)[C@H](N6C=NC7=C6N=C(N)NC7=O)O5 | ||
| 分子式 | C20H24N10O13P2 | 分子量 | 674.41 |
| 溶解度 | Water : 20 mg/mL (29.66 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.4828 mL | 7.4139 mL | 14.8278 mL |
| 5 mM | 296.6 μL | 1.4828 mL | 2.9656 mL |
| 10 mM | 148.3 μL | 741.4 μL | 1.4828 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
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