CellFluor? GST is a cell-permeable quenched fluorescent probe for the detection of pan-glutathione S-transferase (GST) activity in adherent or suspension cells, cell or tissue lysates, or purified enzyme preparations. It is composed of rhodamine 110 protected by the GST substrate dinitrobenzenesulfonamide (DNB).1,2 Upon deprotection by GSTs, rhodamine 110 is released, and its fluorescence can be quantified using excitation/emission maxima of 496/520 nm, respectively.
References:
[1]. Shibata, A., Furukawa, K., Abe, H., et al.Rhodamine-based fluorogenic probe for imaging biological thiolBioorg. Med. Chem. Lett.18(7)2246-2249(2008).
[2]. ?lander, J., Johansson, K., Heuser, V.D., et al.Characterization of a new fluorogenic substrate for microsomal glutathione transferase 1Anal. Biochem.390(1)52-56(2009).
















