CCVJ is a fluorescent molecular rotor whose fluorescence intensity (excitation wavelength 420nm, emission wavelength 491nm) is inversely proportional to its intramolecular rotation rate. CCVJ can non-covalently bind to hydrophobic pockets of proteins via its large hydrophobic structure, and CCVJ fluorescence is significantly enhanced when increased viscosity inhibits intramolecular rotation. CCVJ is suitable for monitoring changes in solution or membrane viscosity, polymerization processes, and protein conformational dynamics[1-2].
References:
[1] Zeng Y, Cheng Y, Tang N. Rapid determination of amylopectin content in starch using molecular rotor fluorescent probes. Food Chem. 2025 Aug 15;483:144297.
[2] Schmidt MJ, Sauter D, Rösgen T. Flow-dependent fluorescence of CCVJ. J Biol Eng. 2017 Aug 3;11:24. doi: 10.1186/s13036-017-0067-2.
CCVJ是一种荧光分子转子，荧光强度（激发波长420nm、发射波长491nm）与分子内旋转速率成反比。CCVJ可通过较大的疏水结构与蛋白质的疏水口袋非共价结合，并因粘度升高抑制分子内旋转而显著增强荧光。CCVJ适用于监测溶液或膜粘度变化、聚合过程及蛋白质构象动态[1-2]。