Calmodulin-dependent protein kinase II (290-309) is a synthetic peptide derived from the rat brain protein sequence that contains the calmodulin binding domain.1,2 It inhibits calcium/calmodulin-dependent protein kinase II (CaMKII) with an IC50 value of 52 nM and CaMKII-dependent phosphodiesterase activity (IC50 = 1.1 nM). Calmodulin-dependent protein kinase II (290-309) has been used in the study of CaM binding, autophosphorylation, and dynamics.3,4
1.Payne, E.M., Fong, Y.-L., Ono, T., et al.Calcium/Calmodulin-dependent Protein Kinase IIJ. Biol. Chem.263(15)7190-7195(1988) 2.Lin, C.R., Kapiloff, M.S., Durgerian, S., et al.Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinaseProc. Natl. Acad. Sci. USA84(16)5962-5966(1987) 3.Wyttenbach, T., Grabenauer, M., Thalassinos, K., et al.The effect of calcium ions and peptide ligands on the relative stabilities of the calmodulin dumbbell and compact structuresJ. Phys. Chem. B.114(1)437-447(2010) 4.Colbran, R.J., and Soderling, T.R.Calcium/calmodulin-independent autophosphorylation sites of calcium/calmodulin-dependent protein kinase II. Studies on the effect of phosphorylation of threonine 305/306 and serine 314 on calmodulin binding using synthetic peptidesJ. Biol. Chem.265(19)11213-11219(1990)