Calcein Deep Red™ AM is a fluorogenic probe. As the acetoxymethyl ester, Calcein Deep Red™ AM is non-fluorescent but cell-permeant and is hydrolyzed to the fluorescent probe Calcein Deep Red™ by intracellular esterases. Calcein Deep Red™ is retained by live cells and displays excitation/emission maxima of 643/663 nm, respectively.
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