Biotinidase Human Recombinant
Sample solution is provided at 25 µL, 10mM.
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BTD Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 510 amino acids (44-545a.a) and having a molecular mass of 57.8kDa. (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).BTD is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
| Purity | Greater than 85% as determined by SDS-PAGE. | Source | Sf9,Baculovirus cells. |
| Phycical Appearance | Sterile Filtered colorless solution. | Shipping Condition | Shipped with Ice Packs. |
| Synonyms | Biotinidase;EC 3.5.1.12; Biotinase; EC 3.5.1. | ||
| Amino Acid Sequence | AHTGEESVAD HHEAEYYVAA VYEHPSILSL NPLALISRQE ALELMNQNLDIYEQQVMTAA QKDVQIIVFP EDGIHGFNFT RTSIYPFLDF MPSPQVVRWN PCLEPHRFND TEVLQRLSCMAIRGDMFLVA NLGTKEPCHS SDPRCPKDGR YQFNTNVVFS NNGTLVDRYR KHNLYFEAAFDVPLKVDLIT FDTPFAGRFG IFTCFDILFF DPAIRVLRDY KVKHVVYPTAWMNQLPLLAA IEIQKAFAVA FGINVLAANV HHPVLGMTGS GIHTPLESFW YHDMENPKSH LIIAQVAKNPVGLIGAENAT GETDPSHSKF LKILSGDPYC EKDAQEVHCD EATKWNVNAP PTFHSEMMYDNFTLVPVWGK EGYLHVCSNGLCCYLLYERP TLSKELYALG VFDGLHTVHG TYYIQVCALV RCGGLGFDTC GQEITEATGI FEFHLWGNFS TSYIFPLFLTSGMTLEVPDQ LGWENDHYFL RKSRLSSGLV TAALYGRLYE RDLEHHHHHH. | ||
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. | ||
| Formulation | BTD protein solution (0.25mg/ml) containing PhosphateBuffered Saline (pH 7.4) and 10% glycerol. | ||
Biotinidase also known BTD, belongs to the nitrilasesuperfamily, which contains 12 families of nitrilases, amidases, carbamylases,and N-acyltrasferases. BTD catalyzes the hydrolysis of biocytin, the product ofbiotin-dependent carboxylase degradation, to biotin and lysine. BTD has a vitalregulatory part in chromatin/DNA function. Mutationsin BTD protein lead to Biotinidase deficiency.
Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.