BSA control for BSA-fatty acid complexes (5 mM) is a fatty acid-free preparation of BSA intended for use as a control for BSA-fatty acid complexes containing 5 mM fatty acid (Cas No. GC46103). It can be used as a control for experiments using BSA-fatty acid complexes to deliver fatty acids to cells in culture for the purpose of monitoring fatty acid oxidation or similar processes in various cellular metabolic studies.[1,2,3] The BSA/BSA-FAs are acceptable for use with short term cell culture applications (acute treatment to 18 hours), however for long term 25+ hours the product can/should be filter sterilized using a 0.2 µm filter and sterile receptacle without affecting the performance.
Formulation: 0.8 mM BSA in 150 mM sodium chloride, pH 7.4
References:
[1]. Alsabeeh, N., Chausse, B., Kakimoto, P.A., et al. Cell culture models of fatty acid overload: Problems and solutions. Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1863(2), 143-151 (2018).
[2]. Wang, D., Green, M.F., McDonnell, E., et al. Oxygen flux analysis to understand the biological function of sirtuins. Methods Mol. Biol. 1077, 241-258 (2013).
[3]. Bentebibel, A., Sebastián, D., Herrero, L., et al. Novel effect of C75 on carnitine palmitoyltransferase I activity and palmitate oxidation. Biochemistry 45(14), 4339-4350 (2006).