Animal experiment: | Mice, Rats, Dogs and Ferrets[1]Swiss albino mice (24-28 g, males age 4 weeks, females age 6 weeks; pregnant mice mated at 6 weeks, used at day 18 of pregnancy) and Wistar albino rats (200 g males age 6 weeks, females age 8 weeks; 350 g males age 10 weeks) are fed Dixon's mouse and rat diet and have free access to water. Albino ferrets (0.7-2.6 kg, age 9-15 months) are fed raw meat, bread and milk. Male beagles (10.8-12.3 kg, age 10-14 years) are fed Spratt's complete dog diet. [3H]Bifluranol administration is by intragastric intubation, in propylene glycol (mouse 0.1 ml, rat 0.1-0.2 mL, ferret 0.1-0.4 mL and dog 1 mL), except for the dog 96 h excretion study when the drug is absorbed onto starch and given in a gelatin capsule. Bifluranol is given intravenously in propylene glycol-0.9% NaCl (saline) (1 : 1 v/v) (0.1-0.2 mL), via a tail vein in mice and rats and the jugular vein in ferrets (under ether anaesthesia). [3H]Bifluranol (2 mg/kg, 1.1 mCi) is administered orally or intravenously to male, female and pregnant mice. After various time intervals they are killed under ether anaesthesia by immersion in solid CO2-hexane (-70°C). The tail, limbs and ears are removed. The animals shaved, embedded and frozen in 5 % aq. acacia wax. The animal blocks are cut using a Slee whole-body freezing microtome to obtain lateral sections (30 pm) which are exposed to X-ray film at 4°C and the auto-radiograms examined after 1,3 or 6 months. [3H]Bifluranol is adrninistered orally or intravenously to rats (200 μg/kg, 0.86-1.0 mCi) , ferrets (60 μg/kg, 5.0-10.6 mCi) and orally only to dogs (50 μg/kg, 70-76 mCi) . Blood samples (10-100 μL) are taken for radioactivity determination at time intervals up to 96 h (rat and ferret) or 6 h (dog). |
References: [1]. Pope DJ, et al. Bifluranol, a novel fluorinated bibenzyl anti-androgen, its chemistry and disposition in different animal species. J Pharm Pharmacol. 1981 May;33(5):297-301. |
