BBR-BODIPY is a fluorescent probe that allows screening its interaction with the targeted cells. BBR-BODIPY induces apoptosis and changes the expression of apoptosis-related proteins.
BBR-BODIPY (1-32 μM; 24 hours; MCF7 and MDA-MB-231 cells) has anti-proliferative activity[1].
BBR-BODIPY (2-16 μM; 5-60 mins; MCF7 cells) could be quickly taken up into MCF7 cells in 5 mins and the fluorescence intensity exhibits a time-dependent manner[1].
BBR-BODIPY (1-32 μM; 24 hours; MCF7 and MDA-MB-231 cells) accumulates in mitochondria and overlaps with Mito tracker red to display yellow fluorescence with a Pearsons co-localization coefficient of 0.72[1].
BBR-BODIPY (8 μM, 12 hours) induces apoptosis in MCF7 cells through a mitochondrial pathway[1].
References:
[1]. Jin M, et al. Synthesis of a novel fluorescent berberine derivative convenient for its subcellular localization study. Bioorg Chem. 2020 Aug;101:104021.