Avobenzone-13C-d3 is intended for use as an internal standard for the quantification of avobenzone by GC- or LC-MS. Avobenzone is a full-spectrum ultraviolet A (UVA) blocker.1 It inhibits UVA-induced increases in melanin levels and tyrosinase activity in B16/F10 melanoma cells (IC30s = 21.94 and 24.25 μM, respectively).2 Avobenzone (30 μM) also inhibits UVA-induced production of reactive oxygen species (ROS) and 8-hydroxy-2'-deoxyguanosine , as well as inhibits UVA-induced depletion of glutathione (GSH), in B16/F10 cells. It increases nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) and upregulates the antioxidant response element (ARE) in UVA-irradiated B16/F10 cells when used at at a concentration of 30 μM. Formulations containing avobenzone have been used as sun protectants in sunscreen products.
1.Beasley, D.G., and Meyer, T.A.Characterization of the UVA protection provided by avobenzone, zinc oxide, and titanium dioxide in broad-spectrum sunscreen productsAm. J. Clin. Dermatol.11(6)413-421(2010) 2.Chaiprasongsuk, A., Onkoksoon, T., Pluemsamran, T., et al.Photoprotection by dietary phenolics against melanogenesis induced by UVA through Nrf2-dependent antioxidant responsesRedox Biol.879-90(2016)