ATP-Red 1 is a switchable, multi-site fluorescent probe designed for rapid and selective monitoring of ATP (adenosine triphosphate) concentration changes in living cells (Ex/Em = 510/590nm) [1]. When unbound to ATP, ATP-Red 1 exhibits a closed ring structure and is virtually non-fluorescent. Upon ATP binding, the ring opens, emitting red fluorescence, clearly reflecting ATP levels [2-3].
References:
[1]. Wang L, Yuan L, Zeng X, et al. A multisite‐binding switchable fluorescent probe for monitoring mitochondrial ATP level fluctuation in live cells[J]. Angewandte Chemie International Edition, 2016, 55(5): 1773-1776.
[2]. Fiorillo M, Ózsvári B, Sotgia F, et al. High ATP production fuels cancer drug resistance and metastasis: implications for mitochondrial ATP depletion therapy[J]. Frontiers in oncology, 2021, 11: 740720.
[3]. Ren T B, Wen S Y, Wang L, et al. Engineering a reversible fluorescent probe for real-time live-cell imaging and quantification of mitochondrial ATP[J]. Analytical Chemistry, 2020, 92(6): 4681-4688.
ATP-Red 1是一种可切换的多位点荧光探针，用于快速、选择性地监测活细胞中ATP（三磷酸腺苷）浓度的变化（激发/发射波长= 510/590nm） [1]。未与ATP结合时，ATP-Red 1呈现闭环结构，几乎不发荧光。与ATP结合后，环打开，发出红色荧光，清晰地反映ATP水平 [2-3]。