Ac-VLPE-FMK, a tetrapeptidyl mono-fluorometl ketone (m-FMK), is a Cat-B and Cat-L inhibitor. Ac-VLPE-FMK can be used for the research of cancer aggressiveness[1][2].
Ac-VLPE-FMK (2 μM) 显着影响人重组 CtsB 和 L 对探针 Ac-PLVQ-AMC 的裂解[1]。
Ac-VLPE-FMK (30 min) 抑制 769-p 和 A498 细胞中底物 Ac-PLVQ-AMC 裂解产生的荧光[1]。
Ac-VLPE-FMK (2.5-250 μM; 24-72 h) 不影响肾癌细胞活力，但影响肾癌细胞的细胞迁移率、细胞粘附、集落形成和标志物表达[1]。
[1]. Rudzińska M, et, al. Cysteine Cathepsins Inhibition Affects Their Expression and Human Renal Cancer Cell Phenotype. Cancers (Basel). 2020 May 21;12(5):1310.
[2]. Citarella A, et, al. Peptidyl Fluorometl Ketones and Their Applications in Medicinal Chemistry. Molecules. 2020 Sep 3;25(17):4031.