Ac-LEHD-pNA is a substrate for caspase-9.1 Caspase-9 preferentially binds to and cleaves the Leu-Glu-His-Asp (LEHD) peptide sequence to release p-nitroalinide, which can be quantified by colorimetric detection at 405 nm as a measure of enzyme activity.1,2,3
1.Hsu, Y.L., Kuo, P.L., Lin, L.T., et al.Asiatic acid, a triterpene, induces apoptosis and cell cycle arrest through activation of extracellular signal-regulated kinase and p38 mitogen-activated protein kinase pathways in human breast cancer cellsJournal of Pharmacology and Experimental Therapeutics313(1)333-344(2005) 2.Por?ba, M., StrÓ?yk, A., Salvesen, G.S., et al.Caspase substrates and inhibitorsCold Spring Harb. Perspect. Biol.5(8)A008680(2013) 3.Yu, M.-S., Suen, K.-C., Kwok, N.-S., et al.Beta-amyloid peptides induces neuronal apoptosis via a mechanism independent of unfolded protein responsesApoptosis11(5)687-700(2006)