9(S)-HETE-d8 is intended for use as an internal standard for the quantification of 9(S)-HETE by GC- or LC-MS. 9(S)-HETE is the (S) isomer of the monohydroxy fatty acid (±)9-HETE .1 It is formed from arachidonic acid by rat liver microsomal cytochrome P450 (CYP).2 9(S)-HETE is an agonist of retinoid X receptor γ (RXRγ), inducing RXRγ-dependent transcription when used at a concentration of 300 nM in BHK cell extracts expressing chick RXRγ.3
1.Schneider, C., Yu, Z., Boeglin, W.E., et al.Enantiomeric separation of hydroxy and hydroperoxy eicosanoids by chiral column chromatographyMethods Enzymol.433145-157(2007) 2.Capdevila, J., Yadagiri, P., Manna, S., et al.Absolute configuration of the hydroxyeicosatetraenoic acids (HETEs) formed during catalytic oxygenation of arachidonic acid by microsomal cytochrome P-450Biochem. Biophys. Res. Commun.141(3)1007-1011(1986) 3.Eager, N.S., Brickell, P.M., Snell, C., et al.Structural and functional evidence for activation of a chick retinoid X receptor by eicosanoidsProc. Biol. Sci.250(1327)63-69(1992)