8-Bromo-cAMP (8-Bromo-cAMP, sodium salt) is a cell-permeable cAMP analogue that acts as a CAMP-dependent protein kinase activator (PKA activator). It has the ability to inhibit the growth of tumor cells and facilitate tumor differentiation [1-2].
8-Bromo-cAMP (20μM;24 h/48h) induced differentiation and apoptosis of human esophageal carcinoma cells Eca-109 [3]. 8-Bromo-cAMP (0.1/0.5 mM;10days) enhances the efficiency of inducing pluripotency in human fibroblast cells[4]. 8-Bromo-cAMP (250 μM) inhibits glucose transport activity in mouse placental cells in culture [5]. 8-Bromo-cAMP(0-1mM) induces a proliferative response in an IL-3 dependent leukemic cell line[6].
8-Bromo-cAMP (60 mg/kg/ day; 7 days; i.p) significantly inhibited the growth of CT26 tumor in mice[7]. 8-Bromo-cAMP enhances both humoral and cell-mediated immune responses induced by an HIV-1 DNA vaccine in mice[8].
References:
[1]. Chen TC, Hinton DR, et,al. Up-regulation of the cAMP/PKA pathway inhibits proliferation, induces differentiation, and leads to apoptosis in malignant gliomas. Lab Invest. 1998 Feb;78(2):165-74. PMID: 9484714.
[2]. Li H, Chen HC, et,al.Identification of a rapidly dephosphorylating 95-kDa protein as elongation factor 2 during 8-Br-cAMP treatment of N1E115 neuroblastoma cells. Biochem Biophys Res Commun. 1995 Dec 5;217(1):131-7. doi: 10.1006/bbrc.1995.2754. PMID: 8526900.
[3]. Wang HM, Zheng NG, et,al. Dual effects of 8-Br-cAMP on differentiation and apoptosis of human esophageal cancer cell line Eca-109. World J Gastroenterol. 2005 Nov 7;11(41):6538-42. doi: 10.3748/wjg.v11.i41.6538. PMID: 16425431; PMCID: PMC4355801.
[4]. Wang Y, Adjaye J. A cyclic AMP analog, 8-Br-cAMP, enhances the induction of pluripotency in human fibroblast cells. Stem Cell Rev Rep. 2011 Jun;7(2):331-41. doi: 10.1007/s12015-010-9209-3. PMID: 21120637.
[5]. Sakata M, Yamaguchi M, et,al. 8-Bromo-cAMP inhibits glucose transport activity in mouse placental cells in culture. J Endocrinol. 1996 Aug;150(2):319-27. doi: 10.1677/joe.0.1500319. PMID: 8869598.
[6]. Barge RM, Falkenburg JH, et,al. 8-Bromo-cAMP induces a proliferative response in an IL-3 dependent leukemic cell line and activates Erk 1,2 via a Shc-independent pathway. Biochim Biophys Acta. 1997 Feb 4;1355(2):141-6. doi: 10.1016/s0167-4889(96)00130-9. PMID: 9042334.
[7]. Wang S, Zhang Z, et,al. Angiogenesis and vasculogenic mimicry are inhibited by 8-Br-cAMP through activation of the cAMP/PKA pathway in colorectal cancer. Onco Targets Ther. 2018 Jul 2;11:3765-3774. doi: 10.2147/OTT.S164982. PMID: 29997437; PMCID: PMC6033084.
[8]. Arai H, Xin KQ, et,al. 8 Br-cAMP enhances both humoral and cell-mediated immune responses induced by an HIV-1 DNA vaccine. Gene Ther. 2000 Apr;7(8):694-702. doi: 10.1038/sj.gt.3301145. PMID: 10800093.
8-Bromo-cAMP (8-Bromo-cAMP, sodium salt)是一种细胞渗透性cAMP类似物，是cAMP依赖性蛋白激酶激活剂（PKA激活剂）,它能抑制肿瘤细胞生长，促进肿瘤分化[1-2]。
8-Bromo-cAMP (20μM;24 h/48h)诱导人食管癌细胞Eca-109分化和凋亡[3]。8-Bromo-cAMP (0.1/0.5 mM;10days)可提高人成纤维细胞诱导多能性的效率[4]。8-Bromo-cAMP (250 μM)在培养小鼠胎盘细胞中抑制葡萄糖转运活性[5]。8-Bromo-cAMP (0-1mM)在IL-3依赖性白血病细胞系中诱导增殖反应[6]。
8-Bromo-cAMP (60 mg/kg/ day; 7 days; i.p)显著抑制小鼠CT26肿瘤的生长[7]。8-Bromo-cAMP可增强小鼠HIV-1 DNA疫苗诱导的体液和细胞介导的免疫反应[8]。