7-Azido-4-methylcoumarin is a highly sensitive and selective fluorescent hydrogen sulfide probe (excitation wavelength: 365nm; emission wavelength: 450nm). In the presence of H₂S, the aromatic azido moiety of 7-Azido-4-methylcoumarin can be selectively reduced. 7-Azido-4-methylcoumarin can participate in copper-catalyzed azide-alkyne cycloaddition (CuAAC) and strain-promoted azide-alkyne cycloaddition (SPAAC). 7-Azido-4-methylcoumarin can be used in research related to hydrogen sulfide detection and PLP-dependent enzyme activity monitoring[1-4].
References:
[1] Thorson MK, Majtan T, Kraus JP, et al. Identification of cystathionine β-synthase inhibitors using a hydrogen sulfide selective probe. Angew Chem Int Ed Engl. 2013 Apr 22;52(17):4641-4.
[2] Módis K, Coletta C, Asimakopoulou A, et al. Effect of S-adenosyl-L-methionine (SAM), an allosteric activator of cystathionine-β-synthase (CBS) on colorectal cancer cell proliferation and bioenergetics in vitro. Nitric Oxide. 2014 Sep 15;41:146-56.
[3] Wang H, Jia M, Chang Y, et al. Hydrogen sulfide donor NaHS inhibits formaldehyde-induced epithelial-mesenchymal transition in human lung epithelial cells via activating TGF-β1/Smad2/3 and MAPKs signaling pathways. Curr Res Toxicol. 2024 Oct 24;7:100199.
[4] Chang GQ, Bai SZ, Sun FQ, et al. SKF38393 prevents high glucose (HG)-induced endothelial dysfunction by inhibiting the effects of HG on cystathionine γ-lyase/hydrogen sulfide activity and via a RhoA/ROCK1 pathway. Front Biosci (Landmark Ed). 2022 Feb 11;27(2):49.
7-Azido-4-methylcoumarin是一种高灵敏度和选择性的荧光硫化氢探针（激发波长：365nm；发射波长：450nm）。在H2S存在下，7-Azido-4-methylcoumarin芳香叠氮部分可被选择性还原。7-Azido-4-methylcoumarin可以参与铜催化的叠氮-炔环加成反应（CuAAc），和炔-叠氮环加成反应（SPAAC）。7-Azido-4-methylcoumarin可用于硫化氢检测和PLP依赖性酶活性监测的相关研究[1-4]。