6-Bnz-cAMP is a PKA-selective activator. It regulates the PKA dependent signaling pathways.
Like PKA contains an evolutionally conserved cAMP-binding domain that acts as a molecular switch for sensing intracellular second messenger cAMP levels to control diverse biological functions. CAMP response element-binding protein (CREB) is the well-known direct target protein of PKA. Phosphorylation of CREB (pCREB) by PKA has been shown to be involved in regulating osteoblast differentiation.
The proliferative signaling pathway which activated by the 6-Bnz-cAMP involves activation of the epidermal growth factor receptor and ERK1/2. Extending the duration of PKA-dependent ERK1/2 activation and converted cAMP from a proliferative into an anti-proliferative, neurite outgrowth- promoting signal.
6-Bnz-cAMP can promote not only differentiation and mineralization, but also initial cell adhesion.6-Bnz-cAMP is able to induce osteogenic differentiation of MC3T3-E1 cells. Moreover 6-Bnz-cAMP may facilitate release kinetic from a tissue-engineered polymeric scaffold system. It also can serve as a novel bone-inducing growth factor for repairing and regenerating bone tissues during bone regenerative engineering.
References:
[1]Lo KW, Kan HM, Ashe KM, Laurencin CT. The small molecule PKA-specific cyclic AMP analogue as an inducer of osteoblast-like cells differentiation and mineralization. J Tissue Eng Regen Med. 2012 Jan;6(1):40-8.
[2]Simone Kiermayer, Ricardo M. Biondi,etal., Epac Activation Converts cAMP from a Proliferative into a Differentiation Signal in PC12 Cells. Molecular Biology of the Cell. Vol. 16, 5639–5648, December 2005.
[3]Cheng X, Ji Z, Tsalkova T, Mei F. Epac and PKA: a tale of two intracellular cAMP receptors. Acta Biochim Biophys Sin (Shanghai). 2008 Jul;40(7):651-62.