5’-Deoxyadenosine is an analog of adenosine and an intermediate in the degradation of S-adenosylmethionine (SAM).1 It has been used in the study of enzyme kinetics, including those of phosphomethylpyrimidine synthase, glutamate mutase, and 5’-methylthioadenosine phosphorylase.2,3,4
1.Grove, T.L., Lee, K.-H., St. Clair, J., et al.In vitro characterization of AtsB, a radical SAM formylglycine-generating enzyme that contains three [4Fe-4S] clustersBiochemistry47(28)7523-7538(2008) 2.Palmer, L.D., and Downs, D.M.The thiamine biosynthetic enzyme ThiC catalyzes multiple turnovers and is inhibited by S-adenosylmethionine (AdoMet) metabolitesJ. Biol. Chem.288(42)30693-30699(2013) 3.Chen, H.P., and Marsh, E.N.Adenosylcobalamin-dependent glutamate mutase: Examination of substrate and coenzyme binding in an engineered fusion protein possessing simplified subunit structure and kinetic propertiesBiochemistry36(48)14939-14945(1997) 4.Savarese, T.M., Crabtree, G.W., and Parks, J., R.E.5'-Methylthioadenosine phosphorylase-I substrate activity of 5'-deoxyadenosine with the enzyme from sarcoma 180 cellsBiochem. Pharmacol.30(3)189-199(1981)