4-pentynoyl-Coenzyme A (trifluoroacetate salt)

Protein acetylation is a reversible, post-translational modification regulated by lysine acetyltransferases and deacetylases and plays a key role in regulating gene expression. 4-pentynoyl-Coenzyme A (trifluoroacetate salt) (4-pentynoyl-CoA (trifluoroacetate salt)) is an alkyne-derivatized acetyl-CoA reporter that enables rapid detection of acetylated proteins both in vivo and in vitro. As an acyl-CoA donor, the 4-pentynoyl analog is metabolically transferred onto lysine residues of proteins by lysine acetyltransferases. An azide-alkyne bioconjugation reaction, known as click chemistry, can then be used to readily tag acetylated proteins with fluorescent or biotinylated labels for subsequent analysis.