4-Nitrophenyl-N-acetyl-β-D-glucosaminide is a chromogenic substrate for N-acetyl-β-glucosaminidase.1,2 Hydrolysis of 4-nitrophenyl-N-acetyl-β-D-glucosaminide by N-acetyl-β-glucosaminidase releases 4-nitrophenol, which can be quantified by colorimetric detection at 405 nm as a measure of N-acetyl-β-glucosaminidase activity. It has been used to quantify the activity of N-acetyl-β-D-glucosaminidase in human serum and urine in parenterally fed patients.3
|1. Borooah, J., Leaback, D.H., and Walker, P.G. Studies on glucosaminidase. 2. Substrates for N-acetyl-β-glucosaminidase. Biochem. J. 78(1), 106-110 (1961).|2. Bowers, G.N., Jr., McComb, R.B., Christensen, R.G., et al. High-purity 4-nitrophenol: Purification, characterization, and specifications for use as a spectrophotometric reference material. Clin. Chem. 26(6), 724-729 (1980).|3. Raczkowska, K., Zalewska-Szajda, B., Raczkowski, K., et al. The activity of N-acetyl-β-D-hexosaminidase in serum and urine of parenterally fed patients. Exp. Clin. Hepatology 9, 1-4 (2013).