1-Stearoyl-2-arachidonoyl-d8-sn-glycerol (SAG-d8) is intended for use as an internal standard for the quantification of SAG by GC- or LC-MS. Many protein kinase C (PKC) isoforms require activation via second messengers including Ca2+, diacylglycerol (DAG), and/or a phospholipid in order to phosphorylate target proteins, initiating a variety of important signaling cascades.1 1-Stearoyl-2-arachidonoyl-sn-glycerol (SAG) is a DAG that contains the ω-6 polyunsaturated fatty acid arachidonic acid in the sn-2 position and stearic acid in the sn-1 position of the glycerol backbone. It can potently activate PKCα, ε, and δ at nM concentrations.2 Independent of PKC signaling, SAG competitively binds to the Ras activator RasGRP with a Ki value of 4.49 µM in Jurkat T cells.3
1.Bell, R.M., and Burns, D.J.Lipid activation of protein kinase CThe Journal of Biological Chemisty2664661-4664(1991) 2.Madani, S., Hichami, A., Legrand, A., et al.Implication of acyl chain diacylglycerols in activation of different isoforms of protein kinase CThe FASEB Journal152595-2601(2001) 3.Madani, S., Hichami, A., Charkaoui-Malki, M., et al.Diacylglycerols containing ω-3 and ω-6 fatty acids bind to RasGRP and modulate MAP kinase activationThe Journal of Biological Chemisty279(2)1176-1183(2004)