4-MUNANA (sodium salt) is a fluorescent substrate for neuraminidase activity assays[1]. When 4-MUNANA is hydrolyzed by the enzyme, it releases the fluorophore 4-methylumbelliferyl (4-MU), which has an excitation maximum at 365nm and an emission maximum at 450nm[2, 3]. 4-MUNANA can be used to quantify neuraminidase activity in mammalian cells and clinical isolates of influenza virus[4]. 4-MUNANA can be used to assay sialidase activity[5].
References:
[1]Feng C, Li J, Snyder G, et al. Antibody against microbial neuraminidases recognizes human sialidase 3 (NEU3): the neuraminidase/sialidase superfamily revisited[J]. MBio, 2017, 8(3): 10.1128/mbio. 00078-17.
[2] Singleton E V, Gates C J, David S C, et al. Enhanced immunogenicity of a whole-inactivated influenza A virus vaccine using optimised irradiation conditions[J]. Frontiers in Immunology, 2021, 12: 761632.
[3]Naik N G, Lo Y W, Wu T Y, et al. Baculovirus as an efficient vector for gene delivery into mosquitoes[J]. Scientific reports, 2018, 8(1): 17778.
[4] Wetherall N T, Trivedi T, Zeller J, et al. Evaluation of neuraminidase enzyme assays using different substrates to measure susceptibility of influenza virus clinical isolates to neuraminidase inhibitors: report of the neuraminidase inhibitor susceptibility network[J]. Journal of Clinical Microbiology, 2003, 41(2): 742-750.
[5] Seol B, Kim Y D, Cho Y S. Modeling sialidosis with neural precursor cells derived from patient-derived induced pluripotent stem cells[J]. International Journal of Molecular Sciences, 2021, 22(9): 4386.
4-MUNANA (sodium salt)是一种用于神经氨酸酶活性测定的荧光底物[1]。4-MUNANA被酶水解时，会释放出荧光团4-甲基伞形酮（4-MU），4-MU的最大激发波长为365nm，最大发射波长为450nm[2, 3]。4-MUNANA能够用于定量哺乳动物细胞和流感病毒临床分离株中的神经氨酸酶活性[4]。4-MUNANA能够用于唾液酸酶活性测定[5]。