2-Nitro-5-thiocyanatobenzoic Acid is a highly reactive cyano group donor. 2-Nitro-5-thiocyanatobenzoic Acid can rapidly transfer its cyano group to a nucleophilic thioester, converting the cysteine thiol groups in proteins into S-cyano derivatives, while simultaneously achieving functionalization modification at the C-terminus of proteins through the formation of a 1-acyl-2-iminothiazolidine intermediate. 2-Nitro-5-thiocyanatobenzoic Acid can be used in research related to protein chemical synthesis, cysteine-specific cleavage and labeling, and polypeptide drug development[1-4].
References:
[1] Tang HY, Speicher DW. Identification of alternative products and optimization of 2-nitro-5-thiocyanatobenzoic acid cyanylation and cleavage at cysteine residues. Anal Biochem. 2004 Nov 1;334(1):48-61.
[2] Belghazi M, Klett D, Cahoreau C, et al. Nitro-thiocyanobenzoic acid (NTCB) reactivity of cysteines beta100 and beta110 in porcine luteinizing hormone: metastability and hypothetical isomerization of the two disulfide bridges of its beta-subunit seatbelt. Mol Cell Endocrinol. 2006 Mar 9;247(1-2):175-82.
[3] Koehn H, Clerens S, Deb-Choudhury S, et al. Higher sequence coverage and improved confidence in the identification of cysteine-rich proteins from the wool cuticle using combined chemical and enzymatic digestion. J Proteomics. 2009 Dec 1;73(2):323-30.
[4] Chu Y, Lee EY, Reimann EM, et al. Effect of activation of protein phosphatase 1 on sulfhydryl reactivity. Arch Biochem Biophys. 1996 Oct 1;334(1):83-8.
2-Nitro-5-thiocyanatobenzoic Acid是一种高反应性的氰基供体。2-Nitro-5-thiocyanatobenzoic Acid可迅速将其氰基转移到亲核硫酯上，将蛋白质中的半胱氨酸巯基转化为S-氰基衍生物，同时通过形成1-酰基-2-亚氨基噻唑烷中间体以实现蛋白质碳端的功能化修饰。2-Nitro-5-thiocyanatobenzoic Acid可用于蛋白质化学合成、半胱氨酸特异性切割和标记以及多肽药物开发的相关研究[1-4]。