13-OxoODE-d3

13-OxoODE-d₃ contains three deuterium atoms at the 9, 10, and 12 positions. It is intended for use as an internal standard for the quantification of 13-OxoODE by GC- or LC-mass spectrometry. 13-oxoODE is produced from 13-HODE by a NAD⁺-dependent dehydrogenase present in rat colonic mucosa.¹ 13-OxoODE stimulates cell proliferation when instilled intrarectally in rats.² 13-OxoODE has also been detected in preparations of rabbit reticulocyte plasma and mitochondrial membranes, mostly esterified to phospholipids. Production of 13-oxoODE is putatively linked to the maturation of reticulocytes to erythrocytes through the activity of 15-LO.^3,4

1.Earles, S.M., Bronstein, J.C., Winner, D.L., et al.Metabolism of oxidized linoleic acid: Characterization of 13-hydroxyoctadecadienoic acid dehydrogenase activity from rat colonic tissueBiochimica et Biophysica Acta1081174-180(1991) 2.Bull, A.W., and Bronstein, J.C.Production of unsaturated carbonyl compounds during metabolism of hydroperoxy fatty acids by colonic homogenatesCarcinogenesis111699-1704(1990) 3.KÜhn, H., Belkner, J., Wiesner, R., et al.Occurrence of 9- and 13-keto-octadecadienoic acid in biological membranes oxygenated by the reticulocyte lipoxygenaseArchives of Biochemistry and Biophysics279218-224(1990) 4.KÜhn, H., Belkner, J., and Wiesner, R.Subcellular distribution of lipoxygenase products in rabbit reticulocyte membranesEur. J. Biochem.191(1)221-227(1990)