Z-LLE-AMC is a fluorescent substrate that, when hydrolyzed by specific proteases (20S proteasome, 26S proteasome), releases AMC with blue fluorescence (excitation at 340–360nm, emission at 440–460nm), which is used to monitor protease activity levels[1]. Z-LLE-AMC can be used to detect changes in caspase activity during apoptosis[2]. Z-LLE-AMC can also be used for high-throughput screening of drugs to identify compounds that can modulate protease activity[3].
References:
[1] Tadlock L, Yamagiwa Y, Hawker J, et al. Transforming growth factor-beta inhibition of proteasomal activity: a potential mechanism of growth arrest. Am J Physiol Cell Physiol. 2003 Aug;285(2):C277-85.
[2] Swatek A, Staszczak M. Effect of Ferulic Acid, a Phenolic Inducer of Fungal Laccase, on 26S Proteasome Activities In Vitro. Int J Mol Sci. 2020 Apr 2;21(7):2463.
[3] Barsan MM, Sanz CG, Onea M, et al. Immobilized Antibodies on Mercaptophenylboronic Acid Monolayers for Dual-Strategy Detection of 20S Proteasome. Sensors (Basel). 2021 Apr 12;21(8):2702.
Z-LLE-AMC是一种荧光底物，被特定的蛋白酶水解后，游离的AMC具有蓝色荧光（激发光为340-360nm，发射光为440-460nm），从而用于监测蛋白酶的活性水平[1]。Z-LLE-AMC可用于检测细胞凋亡过程中半胱天冬酶的活性变化[2]。Z-LLE-AMC还可用于高通量筛选药物，寻找能够调节蛋白酶活性的化合物[3]。