Z-Gly-Gly-Arg-AMC is a specific thrombin fluorescent substrate with λexc = 355nm, λem = 460nm[1]. Z-Gly-Gly-Arg-AMC acts as the reference substrate in the Calibrated Automated Thrombogram (CAT) for measuring endogenous thrombin potential (ETP) in platelet-rich (PRP) or platelet-poor plasma (PPP)[2]. Z-Gly-Gly-Arg-AMC is employed in 96-well format for rapid identification and potency ranking of direct thrombin inhibitors or protease-activated receptor antagonists[3].
References:
[1] Tarandovskiy I D, Artemenko E O, Panteleev M A, et al. Antiplatelet agents can promote two-peaked thrombin generation in platelet rich plasma: mechanism and possible applications[J]. PLoS One, 2013, 8(2): e55688.
[2] Machlus K R, Colby E A, Wu J R, et al. Effects of tissue factor, thrombomodulin and elevated clotting factor levels on thrombin generation in the calibrated automated thrombogram[J]. Thrombosis and haemostasis, 2009, 102(11): 936-944.
[3] Kale S S, Bergeron-Brlek M, Wu Y, et al. Thiol-to-amine cyclization reaction enables screening of large libraries of macrocyclic compounds and the generation of sub-kilodalton ligands[J]. Science advances, 2019, 5(8): eaaw2851.
Z-Gly-Gly-Arg-AMC是一种特异性凝血酶荧光底物，激发波长为355nm，发射波长为460nm[1]。Z-Gly-Gly-Arg-AMC作为校准自动化血栓图（CAT）分析中的参照底物，用于测定富血小板血浆或贫血小板血浆中的内源性凝血潜能[2]。Z-Gly-Gly-Arg-AMC可在96孔板体系中用于快速鉴定直接凝血酶抑制剂或蛋白酶激活受体拮抗剂的效价排序[3]。