Thiazole Orange is an asymmetrical cyanine dye belonging to the nucleic acid fluorescent dye family. Thiazole Orange exhibits a significant fluorescence enhancement effect upon binding to nucleic acids, particularly RNA, a property that makes Thiazole Orange widely applicable in biomolecular detection[1]. Thiazole Orange is capable of penetrating the membrane of living cells and demonstrates a high quantum yield upon nucleic acid binding. Thiazole Orange maximum excitation wavelength (λex) is approximately 510nm (with strong excitation also at 488nm and 470nm), and Thiazole Orange maximum emission wavelength (λem) is approximately 527nm[2].
References:
[1] O'Neil CS, Beach JL, Gruber TD. Thiazole orange as an everyday replacement for ethidium bromide and costly DNA dyes for electrophoresis. Electrophoresis. 2018 Jun;39(12):1474-1477.
[2] Lee LG, Chen CH, Chiu LA. Thiazole orange: a new dye for reticulocyte analysis. Cytometry. 1986 Nov;7(6):508-17.
Thiazole Orange是一种不对称的花青素染料，属于核酸荧光染料，与核酸（特别是RNA）结合后会产生显著的荧光增强效应，这一特性使其被广泛应用于生物分子检测[1]。hiazole Orange能够穿透活细胞膜，并且在与核酸结合后表现出高量子产率。其最大激发波长（λex）约为510nm（在488nm和470nm处也有强激发），最大发射波长（λem）约为527nm[2]。