TBHBA (2,4,6-Tribromo-3-hydroxybenzoic acid) is one of the key reagents for the detection of plasma high-density lipoprotein (HDL) cholesterol and uric acid. In the presence of peroxidase, the hydrogen peroxide (H₂O₂) generated during the HDL assay reacts with TBHBA and 4-aminoantipyrine (4-AA) through an oxidative coupling reaction, producing a red quinoneimine dye that exhibits strong absorption at wavelengths of 520–550nm[1].
References:
[1] Trinder P, Webster D. Determination of HDL-cholesterol using 2,4,6-tribromo-3-hydroxybenzoic acid with a commercial CHOD-PAP reagent. Ann Clin Biochem. 1984 Sep;21 ( Pt 5):430-3.
TBHBA (2,4,6-Tribromo-3-hydroxybenzoic acid) TBHBA是检测血浆高密度脂蛋白（HDL）胆固醇和尿酸的关键试剂之一。在过氧化物酶存在下，测定HDL过程中产生的过氧化氢（H₂O₂）能与TBHBA和4-氨基安替比林（4-AA）发生氧化偶联反应，生成在520-550nm波长下有强吸收的红色醌亚胺[1]。