TAMRA azide, 5- isomer is a fluorescent probe that contains an azide group, with the spectral characteristics (excitation 562/40nm, emission 624/40nm)[1]. TAMRA azide, 5-isomer is highly suitable for different click reactions or the Staudinger ligation, and reacting with alkyne, DBCO and BCN[2].
TAMRA azide was used as a fluorophore to label histone H3 and allowed efficient labeling of the modified substrate by CuAAC click chemistry[3]. TAMRA azide has been used for protein microarray immobilization to develop protein biosensors[2]. In HEK293T cells, TAMRA azide has been used in click chemistry to detect S-palmitoylation[4].
References:
[1] Tercel M, McManaway S P, Liyanage H D S, et al. Preparation and properties of clickable amino analogues of the duocarmycins: factors that affect the efficiency of their fluorescent labelling of DNA[J]. ChemMedChem, 2014, 9(9): 2193-2206.
[2] Dadfar S M M, Sekula‐Neuner S, Trouillet V, et al. Protein Microarray Immobilization via Epoxide Ring‐Opening by Thiol, Amine, and Azide[J]. Advanced Materials Interfaces, 2021, 8(10): 2002117.
[3] Willnow S, Martin M, Lüscher B, et al. A selenium‐based click AdoMet analogue for versatile substrate labeling with wild‐type protein methyltransferases[J]. ChemBioChem, 2012, 13(8): 1167-1173.
[4] Yu T, Hou D, Zhao J, et al. NLRP3 Cys126 palmitoylation by ZDHHC7 promotes inflammasome activation[J]. Cell reports, 2024, 43(4).
TAMRA azide, 5- isomer是一种含叠氮基团的荧光探针，具有特定光谱特性（激发波长562/40nm，发射波长624/40nm）[1]。TAMRA azide, 5- isomer非常适用于点击化学反应或施陶丁格连接反应，能与炔烃、DBCO及BCN发生反应[2]。
TAMRA azide可作为组蛋白H3的荧光标记物，通过铜催化叠氮-炔环加成反应（CuAAC）实现修饰底物的高效标记[3]。TAMRA azide已成功应用于蛋白质微阵列固定化技术，以开发蛋白质生物传感器[2]。在HEK293T细胞体系中，TAMRA azide可通过点击化学反应实现S-棕榈酰化修饰的检测[4]。