TAMRA alkyne, 5-isomer is a fluorogenic dye derived from an azide-derivatized tetramethylrhodamine, which can be used to label histone H3 [1]. TAMRA alkyne, 5-isomer is a lipophilic molecule, highly fluorescent, photostable, providing strong signals suitable for detection, and can easily enter the cell by passive diffusion without the need for transporters[2]. TAMRA alkyne, the 5-isomer, can be employed to label the conjugation between azide-functionalized particles and targeting peptides[3].
References:
[1] Willnow S, Martin M, Lüscher B, et al. A selenium‐based click AdoMet analogue for versatile substrate labeling with wild‐type protein methyltransferases[J]. ChemBioChem, 2012, 13(8): 1167-1173.
[2] Kovacs L, Savela R, Matovic J, et al. Development of an In Vitro Assay for Evaluation of Intracellular SPAAC Reactions Using Hoechst-DBCO[J]. Chemical & Biomedical Imaging, 2025.
[3] Ghaffari Sharaf M, Waduthanthri K D, Crichton A, et al. Towards preventing exfoliation glaucoma by targeting and removing fibrillar aggregates associated with exfoliation syndrome[J]. Journal of Nanobiotechnology, 2022, 20(1): 459.
TAMRA alkyne, 5-isomer是一种衍生自叠氮化tetramethylrhodamine的荧光染料，可用于标记组蛋白H3[1]。TAMRA alkyne, 5-isomer是一种亲脂性分子，具有高荧光性、光稳定性，可提供适用于检测的强信号，并且无需转运蛋白即可通过被动扩散轻松进入细胞[2]。TAMRA alkyne, 5-isomer可用于标记叠氮官能化颗粒与靶向肽之间的偶联物[3]。