GlpBio

Janelia Fluor 549, free acid

目录号: GC50479纯度: >98%同义词: JF549 TFA
COASDSData Sheet
Janelia Fluor 549，游离酸 (JF549 TFA) 是一种荧光染料，最大吸收 (Λab (max)) 为 549 nm，发射最大值 (Λem (max)) 为 571 nm。
Janelia Fluor 549, free acid
Cas No.: 2245946-45-4
规格价格库存数量操作
1mg¥1,759.00现货
1
5mg¥3,870.00现货
1
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文献被引

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    Cell
    187(9):2288-2304 (2024)
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    183(7):1867-1883 (2020)
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    33, 904–922 (2023)
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    31, 1291–1307 (2021)

产品描述 Description

Yellow fluorescent dye; supplied as a free acid. Used in protocol (2017 Grimm et al - see references below) for the synthesis of Janelia Fluor® HaloTag® and SNAP-Tag® ligands, for use in live cell imaging experiments. Also suitable for flow cytometry. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1.

Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat. Methods 12 244 PMID:25599551 |Grimm et al (2017) Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments. Methods Mol.Biol. 1663 179 PMID:28924668

产品文档 Product Documents

Purity:>98%
COASDSData Sheet

化学性质Chemical Properties

CAS 号
2245946-45-4
同义词
JF549 TFA
SMILES
OC(C1=CC=C(C(C(C2=CC=C(C=C2OC3=C/4)N5CCC5)=C3C=CC4=[N+]6CCC/6)=C1)C([O-])=O)=O.FC(F)(F)C(O)=O
分子式
C27H22N2O5.C2HF3O2
分子量
568.5 g/mol
溶解性
DMSO : 25 mg/mL (43.98 mM; Need ultrasonic)
保存条件
Store at -20°C
General tips
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
为了提高溶解度，请将管子加热至 37°C，然后在超声波浴中震荡一段时间。
Shipping Condition
评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备 RT，或根据请求配备蓝冰。

计算工具摩尔浓度 / 稀释 / 分子量 / 单位换算 / 体内配方 / 溶解度

g/mol