SYBR Safe DNA Gel Stain is a highly sensitive stain for visualization of DNA in agarose or acrylamide gels. SYBR Safe stain is specifically formulated to be a less hazardous alternative to ethidium bromide that can utilize either blue light or UV excitation.
• Reduce your exposure to highly mutagenic ethidium bromide and harmful UV light
• Increase your sensitivity by reducing nonspecific background fluorescence
• Use in place of ethidium bromide for all staining applications, including RNA staining
A better DNA stain
SYBR Safe DNA Gel Stain is a better nucleic acid staining reagent for all of your molecular biology needs. Not only is SYBR Safe DNA Gel Stain better for you and the environment, it’s better for your sample and your institution.
Safer than ethidium bromide
SYBR Safe DNA Gel Stain has been specifically formulated and evaluated in a battery of toxicity tests with results indicating so that it is less mutagenic and safer for you to work with than ethidium bromide. An independent laboratory showed reduced mutagenicity of SYBR Safe stain compared to ethidium bromide using the Ames test . You can further decrease your exposure risk by using visible blue-light illumination with SYBR Safe stain rather than UV illumination. This is especially valuable when performing exposure-intensive protocols like cutting bands out of gels.
Excellent sensitivity
SYBR Safe stain offers excellent sensitivity in nucleic acid visualization and documentation applications with either UV excitation or blue-light excitation. When bound to nucleic acids, the green-fluorescent SYBR Safe stain has fluorescence excitation maxima at ~280 and ~502 nm, and an emission maximum at ~530 nm (see figure). Plus, when used with blue light illumination, SYBR® Safe stain has less background fluorescence than ethidium bromide–stained gels illuminated with UV light.
Easy to use
SYBR Safe stain is supplied as a 10,000X concentrate in DMSO which can be used just like a solution of ethidium bromide. SYBR Safe stain can be mixed into an agarose gel for staining during electrophoresis or the gel can be incubated in a solution of SYBR Safe stain following electrophoresis. SYBR Safe stain can be stored at room temperature in its original packaging to avoid excessive light exposure.
SYBR Safe DNA Gel Stain是一种高灵敏度的染色剂，用于可视化琼脂或丙烯酰胺凝胶中的DNA。SYBR Safe染料专门设计成比溴化乙锭更安全的替代品，可以使用蓝光或紫外线激发。
• 减少接触高致突变性的溴化乙锭和有害紫外线
• 通过减少非特异性背景荧光来提高灵敏度
• 在所有染色应用中（包括RNA染色）使用SYBR Safe代替溴化乙锭
更好的DNA染料
SYBR Safe DNA Gel Stain是您所有分子生物学需求中更好的核酸染色试剂。不仅对您和环境更好，而且对样本和机构也更好。
比溴化乙锭更安全
SyberSafe DNA Gel Stain经过特别配制并在毒性测试中进行了评估，结果表明其与溴化乙锭相比具有较小突变原性，并且对您工作时更加安全。一个独立实验室显示，在Ames试验中与Ethidium Bromide相比，SyberSafe stain具有降低的突变性。您可以通过使用可见蓝光照明而不是紫外线照明来进一步降低暴露风险，这在执行切割凝胶带等高曝光协议时特别有价值。
优异的灵敏度
SyberSafe染料具有出色的核酸可视化和文档应用程序中的灵敏度，无论是使用紫外线激发还是蓝光激发。当与核酸结合时，绿色荧光SyberSafe染料在约280和502nm处具有荧光激发极大值，并且在约530nm处具有发射极大值（参见图）。此外，在使用蓝光照明时，SyberSafe®染料比用UV照明溴化乙锭染色凝胶具有更少的背景荧光。
易于使用
SyberSafe染料以DMSO 10,000倍浓缩物供应，可以像溴化乙锭溶液一样使用。 SyberSafe染料可以混入琼脂凝胶中进行电泳过程中的着色或者将凝胶浸泡在SyberSafe染料溶液中进行电泳后的着色。 SyberSafe染料可以在室温下存放在其原始包装中，以避免过度照射。