Cy3 NHS ester is a cyanine-containing fluorescent dye commonly used for labeling proteins, antibodies and small molecules, with maximum excitation/emission wavelengths of 550/570nm respectively[1, 2]. Cy3 NHS ester is a high-quality active fluorescent dye optimized for amine labeling. Cy3 dye provides a bright orange signal[1]. Cy3 NHS ester can covalently bind to the primary amine groups (such as lysine) of biological molecules (such as proteins, antibodies, and peptides) through the N-hydroxysuccinimide ester (NHS) group to form a stable amide bond, which is suitable for fluorescent labeling, live cell imaging and molecular detection[3, 4]. The optimal molar ratio of CY dye to protein is about 10:1[5]. The molecular weight of this product is 735.80. It is for research purposes only and is not suitable for food, drugs, medical devices or cosmetics.
References:
[1] Chaudhuri P. AA-Dutp-Cy3 a novel fluorescent labeling agent for nucleotides[J]. Biochemical Pharmacology, 2014, 3: 1000137.
[2] Shan H, Wang N, Gao X, et al. Fluorescent α-Conotoxin [Q1G, ΔR14] LvIB Identifies the Distribution of α7 Nicotinic Acetylcholine Receptor in the Rat Brain[J]. Marine Drugs, 2024, 22(5): 200.
[3] Kang M S, Kong T W S, Khoo J Y X, et al. Recent developments in chemical conjugation strategies targeting native amino acids in proteins and their applications in antibody–drug conjugates[J]. Chemical Science, 2021, 12(41): 13613-13647.
[4] Li Y, Zhou Y, Yue X, et al. Cyanine conjugate‐based biomedical imaging probes[J]. Advanced Healthcare Materials, 2020, 9(22): 2001327.
[5] Pretzer E, Wiktorowicz J E. Saturation fluorescence labeling of proteins for proteomic analyses[J]. Analytical Biochemistry, 2008, 374(2): 250-262.
Cy3 NHS ester是一种含花青（Cyanine）的荧光染料，常用于蛋白、抗体以及小分子化合物的标记，最大激发/发射波长分别为550/570nm[1, 2]。Cy3 NHS ester是针对胺标记优化的高质量活性荧光染料，Cy3染料提供明亮的橙色信号[1]。Cy3 NHS ester能够通过N-羟基琥珀酰亚胺酯（NHS）基团与生物分子（如蛋白质、抗体、多肽）的伯胺基团（如赖氨酸）共价结合，形成稳定的酰胺键，适用于荧光标记、活细胞成像及分子检测[3, 4]。CY染料与蛋白的最佳摩尔比为10:1左右[5]。本产品分子量为735.80，仅供研究用途，不适用于食品、药品、医疗器械或化妆品。