5,6-Dihydrouridine is a pyrimidine nucleoside and derivative of uridine . It is present in, and provides conformational flexibility to, tRNA from bacteria, eukaryotes, and some archaea.1,2 5,6-Dihydrouridine inhibits E. coli cytidine deaminase in a cell-free assay (Ki = 3.4 ?M).3 Serum levels of 5,6-dihydrouridine are increased in patients with prostate cancer and positively correlated with lethality.4
1.Kasprzak, J.M., Czerwoniec, A., and Bujnicki, J.M.Molecular evolution of dihydrouridine synthasesBMC Bioinformatics13153(2012) 2.Dalluge, J.J., Hashizume, T., Sopchik, A.E., et al.Conformational flexibility in RNA: The role of dihydrouridineNucleic Acids Res.24(6)1073-1079(1996) 3.Cohen, R.M., and Woldender, R.Cytidine deaminase from Escherichia coli. Purification, properties and inhibition by the potential transition state analog 3,4,5,6-tetrahydrouridineJ. Biol. Chem.246(24)7561-7656(1971) 4.Huang, J., Mondul, A.M., Weinstein, S.J., et al.Prospective serum metabolomic profiling of lethal prostate cancerInt. J. Cancer145(12)3231-3243(2019)