Suc-Leu-Leu-Val-Tyr-AMC is a fluorogenic substrate for the 20S proteasome, other chymotrypsin-like proteases, and calpains.1,2 Upon enzymatic cleavage, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify protease activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively.
1.Geier, E., Pfeifer, G., Wilm, M., et al.A giant protease with potential to substitute for some functions of the proteasomeScience283(5404)978-981(1999) 2.Sasaki, T., Kikuchi, T., Yumoto, N., et al.Comparative specificity and kinetic studies on porcine calpain I and calpain II with naturally occurring peptides and synthetic fluorogenic substratesJ. Biol. Chem.259(20)12489-12494(1984)