Sphingomyelins是哺乳动物细胞膜中主要的鞘脂类物质，特异性地富集于质膜中。
Cas No.:383907-87-7
Sample solution is provided at 25 µL, 10mM.
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Sphingomyelins are a dominant sphingolipid in membranes of mammalian cells, and this lipid class is specifically enriched in the plasma membrane [1]. Sphingomyelins have a favorable interaction with cholesterol (and other sterols), regulating the distribution of cholesterol within the cell membrane and maintaining the cholesterol homeostasis [2]. Sphingomyelins have been widely used to regulate the lipid mobility of high-density lipoproteins (HDL) and the extracellular flow of cholesterol[3].
In vitro, Sphingomyelins treatment (50µM) for 1 hour significantly promoted the vacuolation in HeLa cells mediated by 10nM vacuolating cytotoxin (VacA)[4].
In vivo, Sphingomyelins treatment via oral administration at a dose of 8mg daily for 7 days aggravated the intestinal mucosal inflammation in a mouse model of colitis induced by dextran sulfate sodium (DSS), and impaired the intestinal mucosal function[5]. Oral administration of 0.05% Sphingomyelins in AIN76A diet for 45 weeks significantly inhibited the progression of colon tumors induced by 1,2-dimethylhydrazine (DMH) in mice[6].
References:
[1] Slotte J P. Biological functions of sphingomyelins[J]. Progress in lipid research, 2013, 52(4): 424-437.
[2] Slotte J P, Ramstedt B. The functional role of sphingomyelin in cell membranes[J]. European journal of lipid science and technology, 2007, 109(10): 977-981.
[3] Martínez-Beamonte R, Lou-Bonafonte J M, Martínez-Gracia M V, et al. Sphingomyelin in high-density lipoproteins: structural role and biological function[J]. International journal of molecular sciences, 2013, 14(4): 7716-7741.
[4] Gupta V R, Patel H K, Kostolansky S S, et al. Sphingomyelin functions as a novel receptor for Helicobacter pylori VacA[J]. PLoS pathogens, 2008, 4(5): e1000073.
[5] Fischbeck A, Leucht K, Frey-Wagner I, et al. Sphingomyelin induces cathepsin D-mediated apoptosis in intestinal epithelial cells and increases inflammation in DSS colitis[J]. Gut, 2011, 60(1): 55-65.
[6] Lemonnier L A, Dillehay D L, Vespremi M J, et al. Sphingomyelin in the suppression of colon tumors: prevention versus intervention[J]. Archives of biochemistry and biophysics, 2003, 419(2): 129-138.
Sphingomyelins是哺乳动物细胞膜中主要的鞘脂类物质，特异性地富集于质膜中[1]。Sphingomyelins与胆固醇（及其他甾醇）具有良好的相互作用，可调节胆固醇在细胞膜内的分布并维持胆固醇稳态[2]。Sphingomyelins已被广泛用于调节高密度脂蛋白的脂质流动性以及胆固醇的细胞外流动[3]。
在体外，50µM的Sphingomyelins处理HeLa细胞1小时，显著促进了10nM空泡毒素介导的细胞空泡化[4]。
在体内，每日口服8mg剂量的Sphingomyelins，连续7天，加剧了葡聚糖硫酸钠(DSS)诱导的结肠炎小鼠模型的肠道黏膜炎症，并损害了肠道黏膜功能[5]。在AIN76A饲料中添加0.05%的Sphingomyelins并每日喂养45周，显著抑制了1,2-dimethylhydrazine (DMH)诱导的小鼠结肠肿瘤的进展[6]。
| Cell experiment [1]: | |
Cell lines | HeLa cells |
Preparation Method | HeLa cells were grown in minimum essential medium (MEM) with 10% (v/v) fetal bovine serum (FBS), 100μg/ml streptomycin, and 100U/ml penicillin at 37°C in 5% CO2/atmosphere. HeLa cells were pretreated for 1h with different concentrations of Sphingomyelins (0,1 5, 10, 25, and 50µM) and were then further incubated at 37°C with 10nM VacA. After 24h, cells were evaluated by DIC microscopy or neutral red uptake assays. DIC images of cells stained with neutral red. |
Reaction Conditions | 0,1 5, 10, 25, and 50µM; 1h |
Applications | Sphingomyelins treatment significantly promoted VacA-mediated vacuolation in HeLa cells in a dose-dependent manner. |
| Animal experiment [2]: | |
Animal models | Female C57-BL/6J-Fue mice |
Preparation Method | Female C57-BL/6J-Fue mice were housed singly in a standard environment with food and water ad libitum. Acute colitis was induced in C57-BL/6 mice with 2.0% DSS over 7 days. 8mg of Sphingomyelins (calculated as 0.2% of the total daily food intake) was resuspended in drinking water and applied by oral gavage for 7 days. Assessment of colonoscopy and histological score in mice. |
Dosage form | 8mg/day for 7 days; p.o. |
Applications | Sphingomyelins treatment significantly aggravated the intestinal mucosal inflammation in mice with DSS-mediated colitis. |
References: | |
| Cas No. | 383907-87-7 | SDF | |
| 别名 | 鞘磷脂(鸡蛋) | ||
| Canonical SMILES | [R]C(N[C@@H](COP(OCC[N+](C)(C)C)([O-])=O)[C@H](O)/C=C/CCCCCCCCCCCCC)=O | ||
| 分子式 | C39H79N2O6P (for palmitoyl) | 分子量 | 703 |
| 溶解度 | Chloroform: Soluble,Ethanol: Warmed,Methanol: Soluble | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.4225 mL | 7.1124 mL | 14.2248 mL |
| 5 mM | 284.5 μL | 1.4225 mL | 2.845 mL |
| 10 mM | 142.2 μL | 711.2 μL | 1.4225 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
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