(±)-Hexanoylcarnitine chloride，酰基肉碱家族的成员之一，是一种脂肪酸代谢物，在细胞能量代谢途径中起重要作用。
Cas No.:6920-35-0
Sample solution is provided at 25 µL, 10mM.
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(±)-Hexanoylcarnitine chloride, a member of the acylcarnitines family, is a fatty acid metabolite that play an important role in cellular energy metabolism pathway[1]. It is commonly used in studies of energy metabolism, mitochondrial function, and peroxisomal β-oxidation activity[1]. (±)-Hexanoylcarnitine chloride can serve as a biomarker for identifying diseases. (±)-Hexanoylcarnitine chloride is significantly and independently associated with mortality in type 2 diabetes and can improve mortality prediction models[2]. (±)-Hexanoylcarnitine chloride, as a specific and easily detectable biomarker, has potential for evaluating skeletal muscle toxicity in rats[3]. (±)-Hexanoylcarnitine chloride can be used as a metabolite standard for the quantitative analysis of various chain-length acylcarnitines by LC-MS/MS[4].
In vitro，Treatment with 4, 8, or 16μM hexanoylcarnitine for 9 days increased the number of CT3 clusters in mouse ESCs undergoing cardiac differentiation in a dose-dependent manner[5].
References:
[1] Dambrova, Maija et al. “Acylcarnitines: Nomenclature, Biomarkers, Therapeutic Potential, Drug Targets, and Clinical Trials.” Pharmacological reviews vol. 74,3 (2022): 506-551.
[2] Scarale MG, Mastroianno M, Prehn C, et al. Circulating Metabolites Associate With and Improve the Prediction of All-Cause Mortality in Type 2 Diabetes. Diabetes. 2022;71(6):1363-1370.
[3] Obayashi H, Kobayashi N, Nezu Y, Yamoto T, Shirai M, Asai F. Plasma 2-hydroxyglutarate and hexanoylcarnitine levels are potential biomarkers for skeletal muscle toxicity in male Fischer 344 rats. J Toxicol Sci. 2017;42(4):385-396.
[4] Giesbertz P, Ecker J, Haag A, Spanier B, Daniel H. An LC-MS/MS method to quantify acylcarnitine species including isomeric and odd-numbered forms in plasma and tissues. J Lipid Res. 2015;56(10):2029-2039.
[5] Tyurin-Kuzmin PA, Molchanov AY, Chechekhin VI, Ivanova AM, Kulebyakin KY. Metabolic Regulation of Mammalian Stem Cell Differentiation. Biochemistry (Mosc). 2020;85(3):264-278.
(±)-Hexanoylcarnitine chloride，酰基肉碱家族的成员之一，是一种脂肪酸代谢物，在细胞能量代谢途径中起重要作用[1]。(±)-Hexanoylcarnitine chloride常用于能量代谢、线粒体功能和过氧化物酶体β-氧化活性的研究[1]。(±)-Hexanoylcarnitine chloride可作为疾病鉴别的生物标志物。(±)-Hexanoylcarnitine chloride与2型糖尿病的死亡率有显著且独立的相关性，并能改善死亡率预测模型[2]。(±)-Hexanoylcarnitine chloride作为特定且易于检测的生物标志物，对于评估大鼠骨骼肌毒性具有重要潜力[3]。(±)-Hexanoylcarnitine chloride可以作为LC-MS/MS定量多种链长酰基肉碱时的代谢物标准品[4]。
体外实验中，4, 8和16μM的(±)-Hexanoylcarnitine chloride持续处理9天，以剂量依赖的方式增加了正在接受心脏分化的小鼠胚胎干细胞中CT3集群的数量[5]。
| Cell experiment [1]: | |
Cell lines | Mouse ESCs undergoing cardiac differentiation |
Preparation Method | The differentiation medium was supplemented with either 4, 8 or 16μM of lauroylcarnitine, (±)-Hexanoylcarnitine chloride, palmitoylcarnitine or ethanol vehicle for 9 days. On day 10, in the 24h before analysis, no supplement was added. Then fixed cells and evaluated them for cardiac differentiation by staining with CT3, an antibody for the cardiac cell marker cardiotroponin, at day 10. |
Reaction Conditions | 4, 8 and 16μM; 9d |
Applications | The addition of each acyl-carnitine increased the number of CT3 clusters as compared to the ethanol-vehicle control. |
References: | |
| Cas No. | 6920-35-0 | SDF | |
| 化学名 | (R)-3-carboxy-2-(hexanoyloxy)-N,N,N-trimethylpropan-1-aminium chloride | ||
| Canonical SMILES | OC(C[C@H](C[N+](C)(C)C)OC(CCCCC)=O)=O.[Cl-] | ||
| 分子式 | C13H26ClNO4 | 分子量 | 295.81 |
| 溶解度 | <29.58mg/ml in Water | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.3805 mL | 16.9027 mL | 33.8055 mL |
| 5 mM | 676.1 μL | 3.3805 mL | 6.7611 mL |
| 10 mM | 338.1 μL | 1.6903 mL | 3.3805 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
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