RS 09

目录号: GC50297纯度: >99.50%

RS 09是一种能够激活TLR-4信号通路的合成肽。RS 09可以作为佐剂增强BALB/c小鼠的免疫反应。

Cas No.: 1449566-36-2

规格	价格	库存	数量
1mg	¥450.00	现货	1
5mg	¥1,080.00	现货	1
10mg	¥1,710.00	现货	1
25mg	¥3,150.00	现货	1

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文献被引

本产品暂无引用记录;以下为 GlpBio 产品在 Nature / Cell / Science 等顶刊的客户引用样例

Nature
641, 529–536 (2025)
Nature
628, 630–638 (2024)
Nature
632, 686–694 (2024)
Nature
618, 1017–1023 (2023)
Nature
610, 366–372 (2022)
Cell
187(9):2288-2304 (2024)
Cell
183(7):1867-1883 (2020)
Science
388(6745) (2025)
Science
387(6739) (2025)
Science
387(6734) (2025)
Cell Research
35, 97–116 (2025)
Cell Research
34, 683–706 (2024)
Cell Research
33, 273–287 (2023)
Cell Research
33, 546–561 (2023)
Cell Research
33, 904–922 (2023)
Cell Research
31, 1291–1307 (2021)

产品描述 Description

RS 09 is a synthetic peptide that can activate the TLR-4 signaling pathway. RS 09 can function as an adjuvant to enhance the immune response in BALB/c mice^[1].

In vitro, RS 09 (10µM; 30min) rescued the suppressed protein levels of TLR4, p-p65/p65, and p-IκBα/IκBα caused by ETS2 deficiency in the inflammatory cell model^[2]. The TLR4 agonist RS 09 (5μg/ml; 24h) impaired the neuroprotective effects of corilagin on N2a cells^[3]. RS 09 (60µM) intervention inhibited anti-inflammatory and pro-M2 polarizing effects of metformin, activating TLR4/NF-κB pathway^[4].

In vivo, In BALB/c mice, when used in combination with the prostate cancer-specific antigen X-15, RS 09 (25µg; 28 days; s.c.) significantly increased the levels of X-15-specific antibodies^[1].

References:
[1] Shanmugam A, Rajoria S, George AL, et al. Synthetic Toll like receptor-4 (TLR-4) agonist peptides as a novel class of adjuvants. PLoS One. 2012;7(2):e30839.
[2] Liu B, Yu J, Zhang J, et al. TLR4/NF-κB-mediated M1 macrophage polarization contributes to the promotive effects of ETS2 on ulcerative colitis. Eur J Med Res. 2025 Jul 24;30(1):668.
[3] Lei Y, Zhou J, Xu D, et al. Corilagin Attenuates Neuronal Apoptosis and Ferroptosis of Parkinson's Disease through Regulating the TLR4/Src/NOX2 Signaling Pathway. ACS Chem Neurosci. 2025 Mar 5;16(5):968-980.
[4] Shen W, Wang Q, Shen G, et al. Regulation of macrophage polarization by metformin through inhibition of TLR4/NF-κB pathway to improve pre-eclampsia. Placenta. 2025 Feb;160:89-99.

RS 09是一种能够激活TLR-4信号通路的合成肽。RS 09可以作为佐剂增强BALB/c小鼠的免疫反应^[1]。

在体外实验中，RS 09（10µM; 30分钟）能够恢复由于ETS2缺失导致的炎症细胞模型中TLR4、p-p65/p65和p-IκBα/IκBα蛋白水平的抑制^[2]。TLR4激动剂RS 09（5µg/ml; 24小时）损害了诃子酸对N2a细胞的神经保护作用^[3]。RS 09（60µM）干预抑制了二甲双胍的抗炎和促M2极化作用，激活了TLR4/NF-κB信号通路^[4]。

在体内实验中，在BALB/c小鼠中，当与前列腺癌特异性抗原X-15联合使用时，RS 09（25µg; 28天; 皮下注射）显著增加了X-15特异性抗体的水平^[1]。

实验参考方法 Experimental Reference Method

Cell experiment [1]:
Cell lines	RAW264.7 cells
Preparation Method	The sh-NC/sh-ETS2 lentivirus was transfected into mouse macrophage RAW264.7 cells for 48h to achieve stable silencing of ETS2. To induce M1 macrophage polarization and establish an inflammatory cell model, the cells were stimulated with lipopolysaccharide (LPS) and interferon-γ (IFN-γ) for 12h. Subsequently, the TLR4 agonist RS 09 (10µM) was added to the cells for 30min to activate the TLR4/NF-κB signaling pathway. The RAW264.7 cells were then collected and resuspended in PBS. Next, 3µL of PE-conjugated iNOS and FITC-conjugated F4/80 antibodies were added, and the cells were incubated in the dark for 20-30min. After another wash, the cells were resuspended in 200µL of PBS, and the proportion of the iNOS+/F4/80+ subgroup was monitored by flow cytometry.
Reaction Conditions	10µM; 30min
Applications	RS 09 rescued the suppressed protein levels of TLR4, p-p65/p65, and p-IκBα/IκBα caused by ETS2 deficiency in the inflammatory cell model.
Animal experiment [2]:
Animal models	Eight-week-old male BALB/c mice
Preparation Method	Eight-week-old male BALB/c mice were divided into four groups (three mice per group). The 15-mer peptide antigen (X-15 peptide) was conjugated to Keyhole Limpet Hemocyanin (KLH) and injected subcutaneously. All mice were vaccinated with 100µg of X-15 conjugated to KLH on day 0 and received a booster of 50µg of X-15 conjugated to KLH on day 14. The difference between the groups was the type of adjuvant used, which was either Alum, RS 01, or RS 09. Alum was added to X-15-KLH at a 1:1 (v:v) ratio, while both RS 01 and RS 09 were added at a concentration of 25µg. OVA peptide (an 8-mer peptide) served as a negative non-adjuvant peptide control and was also used at a concentration of 25µg. Blood was collected on days 0, 14, and 28. The serum collected was used to determine the presence of X-15-specific antibodies via ELISA.
Dosage form	25µg; 28 days; s.c.
Applications	In BALB/c mice, when used in combination with the prostate cancer-specific antigen X-15, RS 09 significantly increased the levels of X-15-specific antibodies.
References: [1] Liu B, Yu J, Zhang J, et al. TLR4/NF-κB-mediated M1 macrophage polarization contributes to the promotive effects of ETS2 on ulcerative colitis. Eur J Med Res. 2025 Jul 24;30(1):668. [2] Shanmugam A, Rajoria S, George AL, et al. Synthetic Toll like receptor-4 (TLR-4) agonist peptides as a novel class of adjuvants. PLoS One. 2012;7(2):e30839.

产品文档 Product Documents

Purity:>99.50%

化学性质Chemical Properties

CAS 号

1449566-36-2

SMILES

[H]N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(O)=O

分子式

C₃₁H₄₉N₉O₉

分子量

691.78 g/mol

溶解性

Water : 100 mg/mL (144.55 mM; Need ultrasonic)

保存条件

Store at -20°C, Sealed, light and moisture resistant storage

General tips

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
为了提高溶解度，请将管子加热至 37°C，然后在超声波浴中震荡一段时间。

Shipping Condition

评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备 RT，或根据请求配备蓝冰。

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